Light-dependent electrochemical properties of the light harvesting complexes of Photosystem II (LHCII) and the corresponding interactions with screen-printed graphite electrodes (GEs) are determined. No exogenous soluble redox mediators are used. LHCII isolated from spinach leaves are immobilized on GE by physical adsorption and through interactions with glutaraldehyde. Importantly, the insertion of LHCII into the pores of a GE is achieved by subjecting the electrode to specific potentials. Both trimeric and aggregated forms of LHCII located within the graphite layer retain their native structures. Voltammetric current peaks centred at ca. −230 and + 50 mV vs. Ag/AgCl (+94 and + 374 mV vs. NHE) limit the investigation of the reduction and oxidation processes of immobilized LHCII. An anodic photocurrent is generated in the LHCII-GE proportional to light intensity and can reach a value of 150 nA/cm². Light-dependent charge separation in LHCII followed by electron transfer to the GE occurs only at potentials of above −200 mV vs. Ag/AgCl (+124 mV vs. NHE). Our results illustrate the importance of the structural proximity of LHCII and GE for photocurrent generation.

确定了光系统II（LHCII）的光收集配合物的光依赖性电化学性质，以及与丝网印刷石墨电极（GE）的相应相互作用。没有使用外源可溶性氧化还原介体。从菠菜叶中分离得到的LHCII通过物理吸附和与戊二醛的相互作用固定在GE上。重要的是，LHCII插入GE孔中是通过使电极经受特定电势来实现的。位于石墨层内的LHCII的三聚体和聚集体形式均保留其天然结构。伏安电流峰值集中在大约 −230和+ 50 mV（相对于Ag / AgCl）（+ 94和+ 374 mV（相对于NHE））限制了固定化LHCII的还原和氧化过程的研究。²。LHCII中依赖光的电荷分离，然后电子转移至GE，仅在相对于Ag / AgCl的电势高于-200 mV（相对于NHE的电势为+124 mV）下发生。我们的结果说明了LHCII和GE的结构邻近性对于光电流产生的重要性。