Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.,Journal of Proteome Research

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Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-02-07 , DOI: 10.1021/acs.jproteome.8b00933
Katie J Lee ₁ , Eithne J Comerford _{1,

2} , Deborah M Simpson ₃ , Peter D Clegg _{1,

2,

4} , Elizabeth G Canty-Laird _{1,

4}

Affiliation

Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options ineffective. Stem cell-based therapies hold potential for treatment of ligament injuries. This study aimed to characterize a ligament progenitor cell (LPC) population and to identify specific niche components which could promote the survival and function of LPCs. LPCs were isolated from canine cranial cruciate ligament and characterized for clonogenicity, multipotency and marker expression. The extracellular matrix (ECM) composition was characterized by the novel application of a metabolic labeling and mass spectrometry technique. LPCs demonstrated clonogenicity, multipotency, and stem cell marker expression. A number of different collagens, glycoproteins, and proteoglycans were identified in the LPC niche using proteomics. Metabolic labeling of cells demonstrated unique turnover profiles for distinct ECM protein groups, indicating the importance of certain niche components for LPC survival and function. The newly synthesized niche components identified in this study could be exploited to aid identification of LPCs and to promote their survival and function for potential ligament repair strategies.

中文翻译：

犬韧带祖细胞及其细胞外基质生态位的鉴定和表征。

韧带在人和动物中容易受伤和退化，但是韧带的愈合潜力很差，当前的治疗选择无效。基于干细胞的疗法具有治疗韧带损伤的潜力。这项研究旨在表征韧带祖细胞（LPC）种群，并确定可以促进LPC存活和功能的特定生态位成分。LPCs是从犬颅十字韧带中分离出来的，并具有克隆形成性，多能性和标志物表达的特征。细胞外基质（ECM）的组成特征是新陈代谢标记和质谱技术的应用。LPC表现出克隆形成性，多能性和干细胞标记物表达。许多不同的胶原蛋白，糖蛋白，使用蛋白质组学技术在LPC生态位中鉴定出了蛋白聚糖。细胞的代谢标记显示了不同ECM蛋白组的独特转换特征，表明某些利基成分对LPC生存和功能的重要性。在这项研究中确定的新合成的利基成分可用于帮助鉴定LPC，并促进其生存和潜在韧带修复策略的功能。

更新日期：2019-02-08

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