Five pairs of enantiomeric lignanamides including nine undescribed compounds along with a known one were obtained from Solanum nigrum L. (Solanaceae). Their structures with absolute configurations were elucidated based on comprehensive spectroscopic analyses and quantum chemical calculations of electronic circular dichroism (ECD) curves. Additionally, all isolates were evaluated for their neuroprotective activity against MPP+ (1-methyl-4-phenylpyridinium)-induced SH-SY5Y cells injury. Among them, cannabisin F showed the most significant neuroprotective effects at different concentrations (12.5, 25, 50 μM). Further studies by Hoechst 33258 staining, monodansylcadaverine (MDC) staining and Annexin V/PI analysis demonstrated that cannabisin F could induce protective autophagy to protect SH-SY5Y cells from MPP+-induced apoptosis.

中文翻译：

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来自 Solanum nigrum L. 的对映体木酚酰胺的表征及其对 MPP+ 诱导的 SH-SY5Y 细胞损伤的神经保护作用

从 Solanum nigrum L.（茄科）中获得了五对对映体木酚酰胺，包括九种未描述的化合物和一种已知的化合物。基于电子圆二色性 (ECD) 曲线的综合光谱分析和量子化学计算，阐明了它们的绝对构型结构。此外，还评估了所有分离株对 MPP+（1-甲基-4-苯基吡啶）诱导的 SH-SY5Y 细胞损伤的神经保护活性。其中，大麻素 F 在不同浓度（12.5、25、50 μM）下表现出最显着的神经保护作用。Hoechst 33258 染色、单丹酰尸胺 (MDC) 染色和膜联蛋白 V/PI 分析的进一步研究表明，大麻素 F 可以诱导保护性自噬，以保护 SH-SY5Y 细胞免受 MPP+ 诱导的细胞凋亡。