Chlorogenic acid relieves lead-induced cognitive impairments and hepato-renal damage via regulating the dysbiosis of the gut microbiota in mice,Food & Function

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Chlorogenic acid relieves lead-induced cognitive impairments and hepato-renal damage via regulating the dysbiosis of the gut microbiota in mice
Food & Function ( IF 5.1 ) Pub Date : 2019-01-18 00:00:00 , DOI: 10.1039/c8fo01755g
Dai Cheng _{1,

2,

3,

4,

5} , He Li _{1,

2,

3,

4,

5} , Jiaping Zhou _{4,

6,

7,

8} , Shuo Wang _{1,

2,

3,

4,

9}

Affiliation

Lead (Pb), a heavy metal which is widely recognized as an environmental toxicant, is transported from the earth's crust into the human body to a significant extent. To control and reduce the hazard of Pb burdens in the human body, chlorogenic acid (CGA) has been used to antagonize Pb-induced cognitive impairments, and hepatic and renal toxicity in the present study. Seven-week-old male Kunming mice were treated with PbCl₂ (1.34 g L⁻¹ in drinking water) and/or CGA (30 mg per kg mouse per day) by gavage administration for 8 weeks. In this study, we evaluated behavior tests, serum biochemical parameters, biomarkers of oxidative stress, and community structure of gut microbiota in mice to explore the potential mechanism of the protective effect. Based on our results, CGA appreciably prevented memory impairment, the release of serum biomarkers, and oxidative stress caused by Pb intake. CGA significantly inhibited Pb-induced increase of cytoplasmic NF-κB, Bax, cytochrome C, and caspase-9 protein expressions. Furthermore, Pb + CGA treatment had a remarkable reversion effect of the gut microbiota composition change induced by Pb, for example increasing the ratio of Helicobacter from 2.95% (Pb) to 11.24% (Pb + CGA) and decreasing the ratio of the Lachnospiraceae_NK4A136_ group from 7.09% (Pb) to 2.68% (Pb + CGA), which suggests that CGA is a superior natural product to eliminate Pb-induced nephrotoxicity and hepatotoxicity.

中文翻译：

绿原酸可通过调节小鼠肠道菌群的营养不良来缓解铅引起的认知障碍和肝肾损害

铅（Pb）是一种被广泛认为是环境有毒物质的重金属，它在很大程度上已从地壳中转移到人体中。为了控制和减少人体中Pb负担的危害，在本研究中，绿原酸（CGA）已被用于拮抗Pb引起的认知障碍以及肝和肾毒性。用PbCl ₂（1.34 g L ^-1和/或CGA（每天每公斤小鼠30 mg）灌胃8周。在这项研究中，我们评估了行为测试，血清生化参数，氧化应激的生物标志物和小鼠肠道菌群的群落结构，以探讨其保护作用的潜在机制。根据我们的结果，CGA可以有效地防止记忆障碍，血清生物标志物的释放以及铅摄入引起的氧化应激。CGA显着抑制Pb诱导的细胞质NF-κB，Bax，细胞色素C和caspase-9蛋白表达的增加。此外，Pb + CGA处理对Pb引起的肠道菌群组成变化具有显着的逆转作用，例如，将幽门螺杆菌的比例从2.95％（Pb）增加到11.24％（Pb + CGA），并降低幽门螺杆菌的比例。Lachnospiraceae_ NK4A136 _从7.09％（PB）至2.68％（铅+ CGA），这表明CGA是一个优越的自然产物，以消除铅诱导的肾毒性和肝毒性基团。

更新日期：2019-01-18

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