Integrative Transcriptome and Proteome Analysis Identifies Major Metabolic Pathways Involved in Pepper Fruit Development.,Journal of Proteome Research

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Integrative Transcriptome and Proteome Analysis Identifies Major Metabolic Pathways Involved in Pepper Fruit Development.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-01-29 , DOI: 10.1021/acs.jproteome.8b00673
Zhoubin Liu _{1,

2} , Junheng Lv _{1,

2} , Zhuqing Zhang ₂ , Heng Li ₃ , Bozhi Yang ₂ , Wenchao Chen ₂ , Xiongze Dai ₂ , Xuefeng Li ₂ , Sha Yang ₂ , Li Liu ₃ , Lijun Ou ₂ , Yanqing Ma ₂ , Xuexiao Zou _{1,

2}

Affiliation

Pepper ( Capsicum annuum L.) fruit development is a complex and genetically programmed process. In this study, we conducted integrative analysis of transcriptome and proteome profiles during pepper fruit development. A total of 23 349 transcripts and 5455 protein groups were identified in four fruit developmental stages of two pepper varieties. The numbers of transcripts and proteins identified were decreased gradually across fruit development, and the most significant changes in transcript and protein levels happened from the mature green (MG) to breaker (Br) stages. Poor correlation between differentially expressed transcript and differentially expressed protein profiles was observed during pepper fruit development. We then analyzed expression profiles of transcripts and proteins involved in cell wall metabolism, and capsanthin, tocopherol, and ascorbate biosynthetic pathways during fruit development, and identified key regulatory proteins in these pathways. We presented a dynamic picture of pepper fruit development via comprehensive analysis of transcriptome and proteome profiles at different fruit developmental stages and in different varieties, revealing the temporal specificity of key protein expression. Our report provides insight into the transcription and translation dynamics of pepper fruit development and a reference for other nonclimacteric species.

中文翻译：

整合转录组和蛋白质组分析确定了辣椒果实发育中涉及的主要代谢途径。

辣椒（Capsicum annuum L.）果实发育是一个复杂的且经过基因编程的过程。在这项研究中，我们进行了辣椒果实发育过程中转录组和蛋白质组图谱的综合分析。在两个辣椒品种的四个果实发育阶段，共鉴定到23 349个转录本和5455个蛋白质组。在整个果实发育过程中，鉴定出的转录本和蛋白质数量逐渐减少，并且转录本和蛋白质水平的最显着变化发生在成熟的绿色（MG）到破碎级（Br）阶段。在辣椒果实发育过程中观察到差异表达的转录本和差异表达的蛋白质谱之间的相关性较差。然后，我们分析了参与细胞壁代谢的转录本和蛋白质以及辣椒红素，生育酚，以及在水果发育过程中抗坏血酸的生物合成途径，并确定了这些途径中的关键调控蛋白。通过综合分析不同果实发育阶段和不同品种的转录组和蛋白质组图谱，我们呈现了辣椒果实发育的动态图景，揭示了关键蛋白质表达的时间特异性。我们的报告提供了对辣椒果实发育的转录和翻译动力学的深入了解，并为其他非更年期物种提供了参考。揭示了关键蛋白表达的时间特异性。我们的报告提供了对辣椒果实发育的转录和翻译动力学的深入了解，并为其他非更年期物种提供了参考。揭示了关键蛋白表达的时间特异性。我们的报告提供了对辣椒果实发育的转录和翻译动力学的深入了解，并为其他非更年期物种提供了参考。

更新日期：2019-02-07

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