A fluorescent sensing platform using KI₃-quenched bovine serum albumin stabilized gold nanoclusters has been designed and used as a fluorescent probe for the turn-on detection of homocysteine/cysteine (Cys/Hcy). The fluorescence of gold nanoclusters was quenched by iodine. The fluorescence of quenched gold nanoclusters was effectively switched on by Cys/Hcy devoid of the interference of glutathione. The transmission electron microscopy image, X-ray photoelectron spectroscopy analysis, time-correlated single photon counting analysis, and dynamic light scattering data confirmed the aggregation-induced quenching of fluorescence of gold nanoclusters by iodine. The turn-on response of Cys/Hcy shows two linear ranges from 0.0057 to 5 μM and from 8 to 25 μM, with a limit of detection of 9 nM for cysteine and 12 nM for homocysteine. Real samples were analyzed to monitor Cys/Hcy added to human serum. The fluorescence turn-on response of the probe on a paper strip in the presence of Cys/Hcy was studied.

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使用KI _3的荧光感应平台淬灭的牛血清白蛋白稳定的金纳米簇已被设计并用作启动检测高半胱氨酸/半胱氨酸（Cys / Hcy）的荧光探针。金纳米团簇的荧光被碘淬灭。Cys / Hcy可有效开启淬灭的金纳米团簇的荧光，而不受谷胱甘肽的干扰。透射电子显微镜图像，X射线光电子能谱分析，与时间相关的单光子计数分析和动态光散射数据证实了碘引起的聚集诱导的金纳米团簇荧光的猝灭。Cys / Hcy的开启响应显示两个线性范围，从0.0057至5μM和从8至25μM，半胱氨酸检测限为9 nM，高半胱氨酸检测限为12 nM。分析真实样品以监测添加到人血清中的Cys / Hcy。研究了在Cys / Hcy存在下，探针在纸条上的荧光开启响应。

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