d- and l-Aspartate regulates growth performance, inflammation and intestinal microbial community in young pigs,Food & Function

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d- and l-Aspartate regulates growth performance, inflammation and intestinal microbial community in young pigs
Food & Function ( IF 5.1 ) Pub Date : 2019-01-11 00:00:00 , DOI: 10.1039/c8fo01410h
Yuying Li _{1,

2,

3,

4,

5} , Hui Han _{1,

2,

3,

4,

5} , Jie Yin _{1,

2,

3,

4,

5} , Xingguo He _{5,

6,

7,

8} , Zhiyi Tang _{1,

2,

3,

4,

5} , Tiejun Li _{1,

2,

3,

4,

5} , Kang Yao _{1,

2,

3,

4,

5} , Yulong Yin _{1,

2,

3,

4,

5}

Affiliation

D-aspartate (D-Asp), an endogenous amino acid, occurs widely in animals and humans with D-enantiomers and plays an important role in the endocrine and nervous systems. However, very few studies are available on growth performance, microbial community, and the intestinal immune and inflammatory status in response to D- and L-aspartate (L-Asp). Thus, in this study, we mainly investigated the effects of dietary 1% D- and L-Asp on growth performance, inflammation, and microbial community in young pigs. Twenty-eight young pigs were randomly divided into four groups (n = 7): a control group, in which piglets were fed a basal diet, and other three groups, in which piglets received 1% D-Asp, 1% L-Asp, and 1% DL-aspartate (DL-Asp) for 35 days. The results showed that dietary 1% D-Asp significantly inhibited average daily feed intake and average daily weight gain. Gut microbes were tested and the results showed that L-Asp enhanced bacterial diversity (Shannon and Simpson). At the phylum level, L-Asp enhanced intestinal Actinobacteria and Bacteroidetes abundance but decreased Firmicutes abundance. In contrast, DL-Asp decreased intestinal Actinobacteria and Bacteroidetes abundance and increased Firmicutes abundance. At the genus level, D-Asp enhanced Clostridium sensu stricto 1 and Intestinibacter abundance. Metagenomic predictions by PICRUSt suggested that the altered microbiota were mainly involved in membrane transport, carbohydrate metabolism, amino acid metabolism, replication and repair, translation, and nucleotide metabolism. In addition, DL-Asp markedly increased the activities of serum alanine aminotransferase, aspartate transaminase and alkaline phosphatase. Also, dietary D- and DL-Asp down-regulated TLR 4, NOD1, and MyD88 in the jejunum to mediate the inflammatory response. Collectively, these results indicated that dietary D-Asp and L-Asp affect the growth performance and inflammation in piglets, which might be associated with gut microbiota.

中文翻译：

d-和l-天门冬氨酸调节幼猪的生长性能，炎症和肠道微生物群落

天冬氨酸D-天冬氨酸（D- Asp）是一种内源性氨基酸，广泛存在于具有D-对映异构体的动物和人类中，并在内分泌和神经系统中起重要作用。然而，很少有研究针对D-和L-天冬氨酸（L -Asp）的生长性能，微生物群落以及肠道免疫和炎症状态。因此，在这项研究中，我们主要研究了日粮1％D-和L -Asp对年幼猪生长性能，炎症和微生物群落的影响。28只幼猪随机分为4组（n= 7）：对照组，其中仔猪接受基础饮食喂养，其他三组，仔猪分别接受1％的D -Asp，1％的L -Asp和1％的DL-天冬氨酸（DL -Asp） 35天结果表明，饮食中1％D -Asp显着抑制了平均日采食量和平均日增重。对肠道细菌进行了测试，结果表明L -Asp可增强细菌多样性（Shannon和Simpson）。在门水平，L -Asp增强肠道放线杆菌和拟杆菌的丰度，但降低Fimicutes的丰度。相反，DL -Asp肠道减少放线菌和拟杆菌的丰度较高，而Fimicutes的丰度较高。在属水平上，D -Asp增强了严格梭状芽胞杆菌1和肠杆菌的丰度。PICRUSt的元基因组学预测表明，改变的微生物群主要涉及膜转运，碳水化合物代谢，氨基酸代谢，复制和修复，翻译和核苷酸代谢。另外，DL- Asp显着增加了血清丙氨酸氨基转移酶，天冬氨酸转氨酶和碱性磷酸酶的活性。另外，饮食中的D-和DL-Asp在空肠中下调TLR 4，NOD1和MyD88介导炎症反应。总的来说，这些结果表明日粮D- Asp和L- Asp会影响仔猪的生长性能和炎症，这可能与肠道菌群有关。

更新日期：2019-01-11

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