Five undescribed cycloartane triterpenoids, including two cycloartane trinor-triterpenoids, were isolated from a 70% ethanol extract of the whole plant of Actaea vaginata (Ranunculaceae), together with thirteen known cycloartane triterpenoids. Their structures were determined by spectroscopic techniques and quantum chemical calculations for intramolecular noncovalent interactions with reduced density gradient method. All compounds were evaluated for their anti-inflammatory effects by a lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production model in RAW264.7 macrophage cells, and some showed potent inhibitory effects with IC50 values ranging from 5.0 to 24.4 μM. Further mechanism studies showed that one compound dose-dependently suppressed LPS-induced NO production and pro-inflammatory cytokines secretion, and decreased the expression of iNOS, through inhibiting NF-κB activation.

中文翻译：

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来自Actaea vaginata 的Cycloartane 三萜类化合物在LPS 刺激的RAW264.7 巨噬细胞中具有抗炎作用

五种未描述的环卡坦三萜类化合物，包括两种环卡坦坦三萜类化合物，是从阴道 Actaea (毛茛科) 全植物的 70% 乙醇提取物中分离出来的，以及 13 种已知的环卡坦三萜。它们的结构是通过光谱技术和量子化学计算确定的，用于分子内非共价相互作用和降低密度梯度方法。在 RAW264.7 巨噬细胞中，通过脂多糖 (LPS) 刺激的一氧化氮 (NO) 产生模型评估了所有化合物的抗炎作用，其中一些化合物显示出有效的抑制作用，IC50 值范围为 5.0 至 24.4 μM。进一步的机制研究表明，一种化合物剂量依赖性地抑制 LPS 诱导的 NO 产生和促炎细胞因子的分泌，