Dietary wood pulp-derived sterols modulation of cholesterol metabolism and gut microbiota in high-fat-diet-fed hamsters,Food & Function

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Dietary wood pulp-derived sterols modulation of cholesterol metabolism and gut microbiota in high-fat-diet-fed hamsters
Food & Function ( IF 5.1 ) Pub Date : 2019-01-09 00:00:00 , DOI: 10.1039/c8fo02271b
Xiang Li _{1,

2,

3,

4,

5} , Huali Wang _{4,

6,

7,

8} , Tianxin Wang _{4,

6,

7,

8} , Fuping Zheng _{3,

4,

5,

9} , Hao Wang _{1,

2,

3,

4,

6} , Chengtao Wang _{1,

2,

3,

4}

Affiliation

Evidence indicates that wood pulp-derived sterols (WS) have beneficial effects on cardiovascular diseases. The present study aimed to (i) investigate the serum cholesterol-lowering activity of dietary WS and (ii) investigate the effects of dietary WS on the balance of gut microbiota in hamsters fed with a high-fat diet. Thirty-six hamsters were divided into four groups fed on a normal chow diet (NCD), a high-fat diet (HFD), or HFD plus 0.1% or 0.5% wood pulp-derived sterols (WSL, WSH), respectively, for 6 weeks. Levels of serum total cholesterol, triglyceride, HDL-C, non-HDL-C, and non-HDL-C/HDL-C ratio in hamsters fed the NCD were originally 120.4 mg dL⁻¹, 235.8 mg dL⁻¹, 71.7 mg dL⁻¹, 48.7 mg dL⁻¹ and 0.68 mg dL⁻¹, which were elevated by being fed the HFD to 187.7 mg dL⁻¹, 389.5 mg dL⁻¹, 92.3 mg dL⁻¹, 95.3 mg dL⁻¹ and 1.03 mg dL⁻¹, and alleviated completely by being fed the WSH. The excretion of total fecal neutral sterols was dose-dependently increased with the amounts of dietary WS. Furthermore, dietary supplementation with WS modulated the relative abundance of gut microbiota compared with the HFD group. Spearman's correlation analysis revealed that Bacteroides, Allobaculum, Coprobacillus, Lactobacillus, Akkermansia, Coprococcus, and Oscillospira were correlated negatively with most of the serum metabolic parameters and cholesterol metabolic parameters, whereas Desulfovibrio was positively correlated with most of the lipid metabolism-associated parameters. Taken together, dietary supplementation with WS was found to have cholesterol-lowering activity, in part mediated by modulating the gut microbiota in a positive way and regulating the cholesterol absorption and metabolism-related genes.

中文翻译：

膳食木浆衍生的固醇对高脂饮食仓鼠胆固醇代谢和肠道菌群的调节

有证据表明，木浆衍生的固醇（WS）对心血管疾病具有有益的作用。本研究旨在（i）研究日粮WS的降低血清胆固醇的活性，以及（ii）日粮WS对高脂饮食仓鼠肠道菌群平衡的影响。将三十六只仓鼠分为四组，分别以普通食物（NCD），高脂饮食（HFD）或HFD加0.1％或0.5％的木浆衍生固醇（WSL，WSH）喂养。 6个星期。血清总胆固醇，甘油三酯水平，HDL-C，非HDL-C，和在仓鼠非HDL-C / HDL-C比供给的NCD最初120.4毫克分升^-1，235.8毫克分升^-1，71.7毫克升^-1，48.7毫克分升^-1和0.68毫克分升^-1，这是由被供给的HFD升高至187.7毫克分升^-1，389.5毫克分升^-1，92.3毫克分升^-1，95.3毫克分升^-1和1.03毫克分升^-1，并通过被供给的WSH完全缓解。粪便中性固醇的排泄量随日粮WS量的增加而呈剂量依赖性增加。此外，与HFD组相比，WS膳食补充剂可调节肠道菌群的相对丰度。Spearman相关分析显示，类杆菌，Allobaculum，Coprobacillus，乳杆菌，Akkermansia，Coprococcus和Oscillospira与大多数血清代谢参数和胆固醇代谢参数呈负相关，而脱硫弧菌与大多数脂质代谢相关参数呈正相关。两者合计，发现通过WS进行膳食补充具有降低胆固醇的活性，部分是通过以积极的方式调节肠道菌群并调节胆固醇吸收和代谢相关基因来介导的。

更新日期：2019-01-09

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