Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs.,Journal of Proteome Research

当前位置： X-MOL 学术 › J. Proteome Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-01-23 , DOI: 10.1021/acs.jproteome.8b00647
Lizandra Jimenez ₁ , Hui Yu ₂ , Andrew J McKenzie ₃ , Jeffrey L Franklin _{1,

4} , James G Patton ₅ , Qi Liu ₆ , Alissa M Weaver _{1,

7}

Affiliation

Extracellular vesicles (EVs) are important mediators of cell-cell communication due to their cargo content of proteins, lipids, and RNAs. We previously reported that small EVs (SEVs) called exosomes promote directed and random cell motility, invasion, and serum-independent growth. In contrast, larger EVs (LEVs) were not active in those assays, but might have unique functional properties. In order to identify protein cargos that may contribute to different functions of SEVs and LEVs, we used isobaric tags for relative and absolute quantitation (iTRAQ)-liquid chromatography (LC) tandem mass spectrometry (MS) on EVs isolated from a colon cancer cell line. Bioinformatics analyses revealed that SEVs are enriched in proteins associated with cell-cell junctions, cell-matrix adhesion, exosome biogenesis machinery, and various signaling pathways. In contrast, LEVs are enriched in proteins associated with ribosome and RNA biogenesis, processing, and metabolism. Western blot analysis of EVs purified from two different cancer cell types confirmed the enrichment of cell-matrix and cell-cell adhesion proteins in SEVs. Consistent with those data, we found that cells exhibit enhanced adhesion to surfaces coated with SEVs compared to an equal protein concentration of LEVs. These data suggest that a major function of SEVs is to promote cellular adhesion.

中文翻译：

小型和大型细胞外囊泡（EVs）的定量蛋白质组学分析揭示了小型EV中粘附蛋白的富集。

细胞外囊泡（EVs）由于其蛋白质，脂质和RNA的货物含量而成为细胞间通信的重要介体。我们之前曾报道过，称为外泌体的小型EV（SEV）促进定向和随机细胞运动，侵袭和血清非依赖性生长。相反，较大的EV（LEV）在这些测定中不起作用，但可能具有独特的功能特性。为了鉴定可能有助于SEV和LEV发挥不同功能的蛋白质货物，我们使用了等压标记对从结肠癌细胞系分离的EV进行了相对和绝对定量（iTRAQ）-液相色谱（LC）串联质谱（MS）。生物信息学分析表明，SEV富含与细胞-细胞连接，细胞-基质粘附，外泌体生物发生机制和各种信号通路相关的蛋白质。相反，LEV富含与核糖体和RNA生物发生，加工和代谢相关的蛋白质。从两种不同癌细胞类型纯化的电动汽车的蛋白质印迹分析证实了SEV中细胞基质和细胞粘附蛋白的富集。与这些数据一致，我们发现与同等浓度的LEV相比，细胞对SEV包被的表面表现出增强的粘附性。这些数据表明，SEV的主要功能是促进细胞粘附。我们发现，与同等浓度的LEV相比，细胞对SEV涂层表面的粘附力增强。这些数据表明，SEV的主要功能是促进细胞粘附。我们发现，与同等浓度的LEV相比，细胞对SEV涂层表面的粘附性增强。这些数据表明，SEV的主要功能是促进细胞粘附。

更新日期：2019-02-07

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11