Ziziphus jujuba Mill. leaves are receiving a great attention for their ability to improve sleep, exerting beneficial effects for both the heart and the central nervous system. With the aim to obtain a comprehensive analysis of the specialised metabolites occurring in the leaves of Z. jujuba, a LC-ESI/(HR)Orbitrap/MS profile in negative ion mode of the methanol extract was carried out. The LC-MS profile guided the isolation of 42 compounds, of which the structures were elucidated by 1D and 2D-NMR experiments. Based on this approach, 2 previously unreported dammarane-type saponins have been characterized along with flavonoid derivatives, dammarane-type saponins and triterpenic acids. Dammarane-type saponins were tested for their cytotoxic activity against A549 and HeLa cancer cell lines. None of the tested compounds, in a range of concentrations between 12.5 and 100 μM, caused a significant reduction of the cell number. This work provides a deep insight on the chemistry of the leaves of Z. jujuba and could be useful for further biological studies aimed at identifying phytochemicals contributing to the health benefits attributed to this herbal drug.

中文翻译：

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LC-ESIMSn 引导下的 Zuziphus jujuba Mill 的深入植物化学分析。树叶

Ziziphus jujuba 磨坊。叶子因其改善睡眠的能力而受到极大关注，对心脏和中枢神经系统都有益处。为了全面分析枣树叶片中的特殊代谢物，我们对甲醇提取物进行了负离子模式下的 LC-ESI/(HR)Orbitrap/MS 分析。LC-MS 谱指导了 42 种化合物的分离，其中的结构通过 1D 和 2D-​​NMR 实验阐明。基于这种方法，2 种以前未报道的达玛烷型皂苷与黄酮类衍生物、达玛烷型皂苷和三萜酸一起被表征。测试了达玛烷型皂苷对 A549 和 HeLa 癌细胞系的细胞毒活性。没有被测试的化合物，在 12.5 和 100 μM 之间的浓度范围内，导致细胞数量显着减少。这项工作提供了对大枣叶子化学的深刻见解，可用于进一步的生物学研究，旨在确定有助于归因于这种草药的健康益处的植物化学物质。