Poly(lactic acid) (PLA) microspheres are widely used in the field of tissue engineering. However, cell adhesion and proliferation on PLA microspheres is limited due to the lack of hydrophilicity and cell recognition sites on microspheres surface. Current methods used to modify PLA microspheres are either complicated or expensive. Aminolysis and hydrolysis are two simple and economical degradation methods. However, up to now, there are few studies using aminolysis or hydrolysis to directly modify PLA microspheres and alter the cell growth behaviour on microspheres. In this study, aminolysis and hydrolysis were used to modify the surface of porous PLA microspheres. After the surface modification, PLA microspheres showed a finely porous structure. Cell culture experiments showed that the modification successfully made the surface of porous PLA microspheres more favourable for cell binding and growth. The increases of porosity, polar groups, hydrophilicity and roughness on microspheres surface were good to cell attachment and proliferation. This study shows that the aminolysis and hydrolysis are simple and effective methods to modify porous PLA microspheres for promoting cell grow on them.

聚乳酸（PLA）微球被广泛用于组织工程领域。然而，由于在微球表面上缺乏亲水性和细胞识别位点，因此在PLA微球上的细胞粘附和增殖受到限制。用于修饰PLA微球的当前方法既复杂又昂贵。氨解和水解是两种简单且经济的降解方法。然而，迄今为止，很少有研究使用氨解或水解直接修饰PLA微球并改变微球上的细胞生长行为。在这项研究中，氨解和水解被用来修饰多孔PLA微球的表面。表面改性后，PLA微球显示出微孔结构。细胞培养实验表明，修饰成功地使多孔PLA微球表面更有利于细胞结合和生长。微球表面孔隙率，极性基团，亲水性和粗糙度的增加有利于细胞的附着和增殖。这项研究表明，氨解和水解是修饰多孔PLA微球以促进细胞在其上生长的简单有效的方法。