Hyaluronic Acid Promotes Differentiation of Mesenchymal Stem Cells from Different Sources toward Pancreatic Progenitors within Three-Dimensional Alginate Matrixes,Molecular Pharmaceutics

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Hyaluronic Acid Promotes Differentiation of Mesenchymal Stem Cells from Different Sources toward Pancreatic Progenitors within Three-Dimensional Alginate Matrixes
Molecular Pharmaceutics ( IF 4.5 ) Pub Date : 2019-01-02 00:00:00 , DOI: 10.1021/acs.molpharmaceut.8b01126
Alberto Cañibano-Hernández _{1,

2} , Laura Saenz del Burgo _{1,

2} , Albert Espona-Noguera _{1,

2} , Gorka Orive _{1,

2} , Rosa M. Hernández _{1,

2} , Jesús Ciriza _{1,

2} , José Luis Pedraz _{1,

2}

Affiliation

Islet transplantation has shown to be a successful alternative in type 1 diabetes treatment, but donor scarcity precludes its worldwide clinical translation. Stem cells are an unlimited source that could circumvent the lack of donors if complete differentiation into insulin-producing cells (IPCs) could be accomplished. We have performed the differentiation of mesenchymal stem cells (MSCs) from different sources into IPCs within three-dimensional (3D) alginate matrixes. We quantified an increased insulin release at the final stage of differentiation compared to undifferentiated MSCs, which is more pronounced in IPCs differentiated from pancreatic-derived MSCs tissues. Moreover, the addition of hyaluronic acid (HA) in alginate microcapsules enhanced, even more, the insulin release from the final IPCs, independent of the MSC source. We can conclude that MSCs can be differentiated into IPCs within alginate microcapsules, enhancing insulin release when HA is present in the 3D alginate matrixes.

中文翻译：

透明质酸促进三维藻酸盐基质内的间充质干细胞从不同来源向胰腺祖细胞的分化。

胰岛移植已被证明是1型糖尿病治疗的一种成功替代方法，但是由于供体的缺乏，其在世界范围内的临床翻译都无法进行。如果可以完全分化为胰岛素产生细胞（IPC），则干细胞是无限来源，可以避免供体的缺乏。我们已经进行了从不同来源的间充质干细胞（MSCs）到三维（3D）海藻酸盐矩阵内的IPC的分化。与未分化的MSC相比，我们在分化的最后阶段定量了增加的胰岛素释放，这在从胰腺来源的MSCs组织分化出来的IPC中更为明显。此外，在藻酸盐微胶囊中添加透明质酸（HA）甚至可以进一步增强最终IPC的胰岛素释放，而与MSC来源无关。

更新日期：2019-01-02

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