Among wild species used in potato breeding, Solanum commersonii displays the highest tolerance to low temperatures under both acclimated (ACC) and non-acclimated (NACC) conditions. It is also the first wild potato relative with a known whole genome sequence. Recent studies have shown that abiotic stresses induce changes in the expression of many small non-coding RNA (sncRNA). We determined the small non-coding RNA (sncRNAome) of two clones of S. commersonii contrasting in their cold response phenotypes via smRNAseq. Differential analysis provided evidence that expression of several miRNAs changed in response to cold stress conditions. Conserved miR408a and miR408b changed their expression under NACC conditions, whereas miR156 and miR169 were differentially expressed only under ACC conditions. We also report changes in tasiRNA and secondary siRNA expression under both stress conditions. Our results reveal possible roles of sncRNA in the regulatory networks associated with tolerance to low temperatures and provide useful information for a more strategic use of genomic resources in potato breeding.

中文翻译：

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Solanum commersonii小RNA文库的深度测序揭示了参与冷应激反应的核糖调节蛋白。

在马铃薯育种中使用的野生物种中，茄子（Solanum commersonii）在适应（ACC）和非适应（NACC）条件下均表现出对低温的最高耐受性。它也是第一个具有已知全基因组序列的野生马铃薯亲戚。最近的研究表明，非生物胁迫导致许多小的非编码RNA（sncRNA）的表达发生变化。我们通过smRNAseq确定了两个S. commersonii克隆的小型非编码RNA（sncRNAome），它们的冷应答表型形成了对比。差异分析提供了证据，表明几种miRNA的表达响应冷胁迫条件而发生了变化。保守的miR408a和miR408b在NACC条件下改变了它们的表达，而miR156和miR169仅在ACC条件下差异表达。我们还报告了在两种压力条件下tasiRNA和二级siRNA表达的变化。我们的结果揭示了sncRNA在与耐低温性相关的调控网络中的可能作用，并为马铃薯育种中基因组资源的更战略性利用提供了有用的信息。