The biological bases of recovery of two grapevine cultivars, Nebbiolo and Barbera, showing different susceptibility and recovery ability to “flavescence dorée” (FD) phytoplasma infection were investigated. The expression over one vegetative season, in FD-recovered and healthy grapevines, of 18 genes involved in defence, hydrogen peroxide and hormone production was verified at two time points. Difference (Δ) between the relative expressions of August and July were calculated for each target gene of both cultivars. The significance of differences among groups assessed by univariate and multivariate statistical methods, and sPLS-DA analyses of the Δ gene expression values, showed that control and recovered grapevines of both cultivars were clearly separated. The Barbera-specific deregulation of defence genes supports a stronger response of this variety, within a general frame of interactions among H₂O₂, jasmonate and ethylene metabolisms, common to both varieties. This may strengthen the hypothesis that FD-recovered Barbera grapevines modulate transcription of their genes to cope with potential damages associated to the alteration of their oxidative status. Nebbiolo variety would fit into this picture, although with a less intense response, in line with its lower degree of susceptibility and recovery incidence to FD, compared to Barbera. The results evidenced a scenario where plant response to phytoplasma infection is highly affected by climatic and edaphic conditions. Nevertheless, even after several years from the original FD infection, it was still possible to distinguish, at molecular level, control and recovered grapevines of both cultivars by analyzing their overall-season response, rather than that of a single time point.

调查了两个葡萄品种Nebbiolo和Barbera恢复的生物学基础，这些生物学基础显示出对“黄花病”（FD）植物质原体感染具有不同的敏感性和恢复能力。在两个时间点验证了在FD恢复和健康的葡萄树中一个植物生长季节中18种涉及防御，过氧化氢和激素产生的基因的表达。对于两个品种的每个靶基因，计算八月和七月的相对表达之间的差异（Δ）。通过单变量和多变量统计方法以及对Δ基因表达值的sPLS-DA分析评估的组之间差异的显着性表明，两个品种的对照和回收葡萄完全分开。₂ O ₂，茉莉花和乙烯代谢，这两个品种共有。这可能会强化以下假设：FD回收的巴贝拉（Barbera）葡萄藤会调节其基因的转录，以应对与其氧化状态改变相关的潜在损害。Nebbiolo品种虽然没有那么强烈的反应，但与Barbera相比，其对FD的敏感性和恢复率较低，因此将适合这种情况。结果证明了这样一种情况，其中植物对植物质体感染的反应受到气候和水生条件的极大影响。但是，即使从最初的FD感染经过数年，仍然有可能通过分析两个品种的总体季节响应，而不是单个时间点，在分子水平上区分两个品种的对照葡萄和恢复的葡萄。