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Smell of Infection: A Novel, Noninvasive Method for Detection of Fish Excretory-Secretory Proteins.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-01-09 , DOI: 10.1021/acs.jproteome.8b00953 Rebecca J Pawluk 1 , Rebekah Stuart 2 , Carlos Garcia de Leaniz 1 , Joanne Cable 3 , Russell M Morphew 4 , Peter M Brophy 4 , Sofia Consuegra 1
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-01-09 , DOI: 10.1021/acs.jproteome.8b00953 Rebecca J Pawluk 1 , Rebekah Stuart 2 , Carlos Garcia de Leaniz 1 , Joanne Cable 3 , Russell M Morphew 4 , Peter M Brophy 4 , Sofia Consuegra 1
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Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on fish hosts are likely to cause release of similar alarm cues into the environment due to the stress, wounding, and immune response stimulated upon infection. Alarm cues are often released in the form of proteins, antimicrobial peptides, and immunoglobulins that provide important insights into bodily function and infection status. Here we outline a noninvasive method to identify potential chemical cues associated with infection in fish by extracting, purifying, and characterizing proteins from water samples from cultured fish. Gel free proteomic methods were deemed the most suitable for protein detection in saline water samples. It was confirmed that teleost proteins can be characterized from water and that variation in protein profiles could be detected between infected and uninfected individuals and fish and parasite only water samples. Our novel assay provides a noninvasive method for assessing the health condition of both wild and farmed aquatic organisms. Similar to environmental DNA monitoring methods, these proteomic techniques could provide an important tool in applied ecology and aquatic biology.
中文翻译：
感染的气味：一种新型的，非侵入性的方法，用于检测鱼的排泄分泌蛋白。
在掠食性攻击或干扰（如寄生虫感染）后，水生生物产生化学信号。以鱼寄主为食的寄生虫可能会因感染引起的压力，创伤和免疫反应而向环境释放类似的警报信号。警报提示通常以蛋白质，抗菌肽和免疫球蛋白的形式释放，这些信息可提供对人体功能和感染状况的重要见解。在这里，我们概述了一种非侵入性方法，可通过从养殖鱼类的水样中提取，纯化和鉴定蛋白质来鉴定与鱼类感染相关的潜在化学线索。无凝胶蛋白质组学方法被认为最适合在盐水样品中检测蛋白质。可以确定硬骨蛋白可以从水中表征，并且可以在感染和未感染的个体以及仅鱼类和寄生虫的水样之间检测到蛋白质分布的变化。我们的新型检测方法为评估野生和养殖水生生物的健康状况提供了一种非侵入性方法。类似于环境DNA监测方法，这些蛋白质组学技术可以为应用生态学和水生生物学提供重要的工具。
更新日期：2019-02-07
中文翻译：
感染的气味：一种新型的，非侵入性的方法，用于检测鱼的排泄分泌蛋白。
在掠食性攻击或干扰（如寄生虫感染）后，水生生物产生化学信号。以鱼寄主为食的寄生虫可能会因感染引起的压力，创伤和免疫反应而向环境释放类似的警报信号。警报提示通常以蛋白质，抗菌肽和免疫球蛋白的形式释放，这些信息可提供对人体功能和感染状况的重要见解。在这里，我们概述了一种非侵入性方法，可通过从养殖鱼类的水样中提取，纯化和鉴定蛋白质来鉴定与鱼类感染相关的潜在化学线索。无凝胶蛋白质组学方法被认为最适合在盐水样品中检测蛋白质。可以确定硬骨蛋白可以从水中表征，并且可以在感染和未感染的个体以及仅鱼类和寄生虫的水样之间检测到蛋白质分布的变化。我们的新型检测方法为评估野生和养殖水生生物的健康状况提供了一种非侵入性方法。类似于环境DNA监测方法，这些蛋白质组学技术可以为应用生态学和水生生物学提供重要的工具。
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