A dozen species of human and animal pathogens have been described to date in the Bordetella genus, with the majority being respiratory tract pathogens. Bordetella avium lipopolysaccharides have been shown to be important virulence factors for this bird pathogen. B. hinzii is closely related to the B. avium species, but has also been isolated from humans. B. trematum is associated to ear and blood infections in humans. Its lipid A structure, the biological active moiety of LPS, was found to be closely related to those of B. avium and B. hinzii. It is important to unveil the subtle structural modifications orchestrated during the LPS biosynthetic pathway to better understand host adaptation. The present data are also important in the context of deciphering the virulence pathways of this important genus containing the major pathogens B. pertussis and B. parapertussis, responsible for whooping cough. We recently reported the isolated lipid A structures of the three presented species, following the previously identified O-chain structures. In the present study, we provide details on the free and O-chain-linked core oligosaccharides which were required to characterize the complete LPS structures. Data are presented here in relation to relevant biosynthesis genes. The present characterization of the three species is well illustrated by Matrix Assisted Laser Desorption Mass Spectrometry experiments, and data were obtained mainly on native LPS molecules for the first time.

迄今为止，在博德特氏菌属中已经描述了十二种人类和动物病原体，其中大多数是呼吸道病原体。业已证明禽博德特氏菌脂多糖是这种禽病原体的重要毒力因子。hinzii B.与鸟形杆菌（B. avium）物种密切相关，但也已从人类中分离出来。银发芽孢杆菌与人类的耳朵和血液感染有关。发现其脂质A结构（LPS的生物活性部分）与禽双歧杆菌和hinzii紧密相关。。重要的是揭示在LPS生物合成途径中精心策划的微妙结构修饰，以更好地了解宿主适应性。在破译含有百日咳博德特氏菌和副百日咳博德特氏菌的这一重要属的毒力途径的背景下，目前的数据也很重要。，引起百日咳。我们最近报告了三个提出的物种，按照先前确定的O链结构分离的脂质A结构。在本研究中，我们提供了表征完整LPS结构所需的游离和O链连接的核心寡糖的详细信息。此处提供有关相关生物合成基因的数据。基质辅助激光解吸质谱实验很好地说明了这三种物质的当前表征，并且首次主要是在天然LPS分子上获得了数据。