In A-to-I RNA editing, adenosine is converted to inosine in double-stranded regions of RNAs. Inosine, an abundant epitranscriptomic mark, contributes to a wide range of biological processes by regulating gene expression post-transcriptionally. To understand the effect of A-to-I RNA editing on regulation of the epitranscriptome, accurate mapping of inosines is necessary. To this end, we established a biochemical method called inosine chemical erasing sequencing (ICE-seq) that enables unbiased and reliable identification of A-to-I RNA editing sites throughout the transcriptome. Here, we describe our updated protocol for ICE-seq in the human transcriptome.

中文翻译：

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使用 ICE-seq 在转录组范围内识别 A-to-I RNA 编辑位点

在 A-to-I RNA 编辑中，腺苷在 RNA 的双链区域被转化为肌苷。肌苷是一种丰富的表观转录组标记，通过在转录后调节基因表达来促进广泛的生物过程。要了解 A-to-I RNA 编辑对表观转录组调控的影响，需要准确定位肌苷。为此，我们建立了一种称为肌苷化学擦除测序 (ICE-seq) 的生化方法，该方法能够在整个转录组中无偏见且可靠地识别 A-to-I RNA 编辑位点。在这里，我们描述了我们在人类转录组中更新的 ICE-seq 协议。