This study evaluated whether resveratrol can alleviate intestinal injury and enhance the mitochondrial function and the mitophagy level in diquat induced oxidative stress of piglets. Twenty-four 35 day old piglets were randomly allocated to four groups: (1) nonchallenged control; (2) control + 100 mg kg⁻¹ resveratrol diet; (3) diquat-treated control and (4) diquat + 100 mg kg⁻¹ resveratrol diet. At the beginning of the experiment, the piglets were injected with diquat or saline (10 mg per kg bodyweight). On day 14, the piglets were killed to obtain the jejuna segments. The results showed that resveratrol increased (P < 0.05) the total antioxidant capacity (T-AOC) and decreased (P < 0.05) the hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) levels in the jejunal mucosa, in comparison with the diquat group. Resveratrol improved (P < 0.05) the intestinal barrier function, as indicated by the increased transepithelial electrical resistance and the decreased paracellular permeability of fluorescein isothiocyanate dextran 4 kDa in the jejunum. Moreover, resveratrol prevented (P < 0.05) the diquat induced decline of occludin, claudin-1 and ZO-1 levels in the jejunal mucosa. Resveratrol ameliorated mitochondria swelling, vacuolation and cracked cristae induced by diquat. Resveratrol decreased (P < 0.05) the ROS generation and increased (P < 0.05) the membrane potential of intestinal mitochondria, the content of mitochondrial DNA and the activity of mitochondrial complexes I–IV in the jejunum. Finally, resveratrol enhanced (P < 0.05) the level of PTEN induced putative kinase 1 (PINK1) and Parkin in intestinal mitochondria; meanwhile it increased (P < 0.05) the LC3-II/LC3-I ratio in the jejunum. These data indicate that resveratrol is effective in protecting the intestinal barrier, improving the redox status, alleviating mitochondrial damage and inducing mitophagy in piglets challenged with diquat.

中文翻译：

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白藜芦醇改善了敌草快致死仔猪的肠屏障功能，减轻了线粒体功能障碍并诱导线粒体吞噬¹

这项研究评估了白藜芦醇是否可以减轻肠道损伤并增强敌草快致仔猪氧化应激的线粒体功能和线粒体水平。将24只35天大的仔猪随机分为4组：（1）无挑战性对照组；（2）对照+ 100 mg kg ^-1白藜芦醇饮食；（3）敌草快处理的对照组和（4）敌草快+ 100 mg kg ^-1白藜芦醇饮食。在实验开始时，给小猪注射敌草快或盐水（每公斤体重10 mg）。在第14天，杀死小猪以获得空肠段。结果表明，白藜芦醇增加（P <0.05）总抗氧化能力（T-AOC），降低（P <0.05）过氧化氢（H ₂与敌草快组相比，空肠黏膜中的O ₂）和丙二醛（MDA）水平。白藜芦醇改善了肠屏障功能（P <0.05），这通过增加空肠中的异硫氰酸荧光素右旋糖苷荧光素4 kDa的跨上皮电阻和降低细胞旁通透性来表明。此外，白藜芦醇可防止（P <0.05）敌草快导致空肠黏膜中occludin，claudin-1和ZO-1水平下降。白藜芦醇减轻了敌草快引起的线粒体肿胀，空泡化和cr裂。白藜芦醇减少（P <0.05）ROS生成并增加（P<0.05）空肠中肠线粒体的膜电位，线粒体DNA的含量以及线粒体复合物I–IV的活性。最后，白藜芦醇增强（P <0.05）肠线粒体中PTEN诱导的假定激酶1（PINK1）和Parkin的水平（P <0.05）；同时，它增加了空肠中LC3-II / LC3-I的比率（P <0.05）。这些数据表明白藜芦醇可有效地保护肠壁屏障，改善氧化还原状态，减轻线粒体损伤并诱导敌草快致死的仔猪的线粒体吞噬。