Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Reversible Mannosylation as a Covalent Binding Adjuvant Enhances Immune Responses for Porcine Circovirus Type 2 Vaccine
ACS Omega ( IF 3.7 ) Pub Date : 2018-12-14 00:00:00 , DOI: 10.1021/acsomega.8b02264 Yuanzi Wu 1 , Chao Yan 1 , Jian’an He 2, 3 , Wenli Xiong 1 , Shuigen Wu 1 , Shutao Liu 1 , Zhen Cai 1
ACS Omega ( IF 3.7 ) Pub Date : 2018-12-14 00:00:00 , DOI: 10.1021/acsomega.8b02264 Yuanzi Wu 1 , Chao Yan 1 , Jian’an He 2, 3 , Wenli Xiong 1 , Shuigen Wu 1 , Shutao Liu 1 , Zhen Cai 1
Affiliation
|
Capsid protein of porcine circovirus type 2 (PCV2) is an ideal subunit vaccine candidate for the postweaning multisystemic wasting syndrome. In this study, mannan-mediated targeting of PCV2ΔCap42-233 protein to antigen presenting cells (APCs) was investigated for the development of PCV2 vaccine. Mannan was attached to PCV2ΔCap42-233 protein via an acid sensitive Schiff base reaction. The mannosylated protein was endowed with the capacity to target the mannose receptor on APCs as well as the ability of controlled release of the antigen in the acidic condition of the lysosome. Finally, the immune response of mannosylated PCV2ΔCap42-233 protein in mice was evaluated. The mannosylated protein exhibited the ability to stimulate humoral immune response and enhance the immunity. Thus, acid-sensitive and APCs-targeting mannosylated PCV2ΔCap42-233 protein represents a promising candidate for the potential commercial application as an efficient vaccine against porcine circovirus.
中文翻译：
可逆甘露糖基化作为共价结合佐剂增强猪圆环病毒2型疫苗的免疫反应。
猪圆环病毒2型衣壳蛋白（PCV2）是断奶后多系统消耗综合症的理想亚单位疫苗候选药物。在这项研究中，研究了甘露聚糖介导的PCV2ΔCap42-233蛋白对抗原呈递细胞（APC）的靶向，以开发PCV2疫苗。甘露聚糖通过对酸敏感的席夫碱反应与PCV2ΔCap42-233蛋白连接。甘露糖基化的蛋白质具有靶向APCs上的甘露糖受体的能力，以及在溶酶体的酸性条件下控制释放抗原的能力。最后，甘露糖基化的PCV2ΔCap42-233的免疫反应评价小鼠中的蛋白质。甘露糖基化的蛋白质表现出刺激体液免疫应答和增强免疫力的能力。因此，酸敏感且靶向APC的甘露糖基化PCV2ΔCap42-233蛋白代表了作为针对猪圆环病毒的有效疫苗的潜在商业应用的有前途的候选者。
更新日期：2018-12-14
中文翻译：
可逆甘露糖基化作为共价结合佐剂增强猪圆环病毒2型疫苗的免疫反应。
猪圆环病毒2型衣壳蛋白（PCV2）是断奶后多系统消耗综合症的理想亚单位疫苗候选药物。在这项研究中，研究了甘露聚糖介导的PCV2ΔCap42-233蛋白对抗原呈递细胞（APC）的靶向，以开发PCV2疫苗。甘露聚糖通过对酸敏感的席夫碱反应与PCV2ΔCap42-233蛋白连接。甘露糖基化的蛋白质具有靶向APCs上的甘露糖受体的能力，以及在溶酶体的酸性条件下控制释放抗原的能力。最后，甘露糖基化的PCV2ΔCap42-233的免疫反应评价小鼠中的蛋白质。甘露糖基化的蛋白质表现出刺激体液免疫应答和增强免疫力的能力。因此，酸敏感且靶向APC的甘露糖基化PCV2ΔCap42-233蛋白代表了作为针对猪圆环病毒的有效疫苗的潜在商业应用的有前途的候选者。
京公网安备 11010802027423号