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Enhanced Uptake of Luminescent Quantum Dots by Live Cells Mediated by a Membrane-Active Peptide.
ACS Omega ( IF 3.7 ) Pub Date : 2018-12-12 , DOI: 10.1021/acsomega.8b02918 Anshika Kapur 1 , Scott H Medina 2 , Wentao Wang 1 , Goutam Palui 1 , Xin Ji 1 , Joel P Schneider 2 , Hedi Mattoussi 1
ACS Omega ( IF 3.7 ) Pub Date : 2018-12-12 , DOI: 10.1021/acsomega.8b02918 Anshika Kapur 1 , Scott H Medina 2 , Wentao Wang 1 , Goutam Palui 1 , Xin Ji 1 , Joel P Schneider 2 , Hedi Mattoussi 1
Affiliation
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The steady progress made over the past three decades in growing a variety of inorganic nanomaterials, with discreet control over their size and photophysical properties, has been exploited to develop several imaging and sensing applications. However, full integration of these materials into biology has been hampered by the complexity of delivering them into cells. In this report, we demonstrate the effectiveness of a chemically synthesized anticancer peptide to facilitate the rapid delivery of luminescent quantum dots (QDs) into live cells. We combine fluorescence imaging microscopy, flow cytometry, and specific endocytosis inhibition experiments to probe QD-peptide conjugate uptake by different cell lines. We consistently find that a sizable fraction of the internalized conjugates does not co-localize with endosomes or the nuclei. These findings are extremely promising for the potential integration of various nanomaterials into biological systems.
中文翻译：
膜活性肽介导的活细胞对发光量子点的吸收增强。
过去三十年来，在生长各种无机纳米材料方面取得了稳步进展，并对其尺寸和光物理特性进行了谨慎控制，已被用于开发多种成像和传感应用。然而，将这些材料完全整合到生物学中受到了将它们输送到细胞中的复杂性的阻碍。在本报告中，我们证明了化学合成的抗癌肽有助于将发光量子点 (QD) 快速传递到活细胞中的有效性。我们结合荧光成像显微镜、流式细胞术和特定的内吞作用抑制实验来探测不同细胞系对 QD 肽缀合物的吸收。我们始终发现，相当大一部分内化缀合物不与内体或细胞核共定位。
更新日期：2018-12-12
中文翻译：
膜活性肽介导的活细胞对发光量子点的吸收增强。
过去三十年来，在生长各种无机纳米材料方面取得了稳步进展，并对其尺寸和光物理特性进行了谨慎控制，已被用于开发多种成像和传感应用。然而，将这些材料完全整合到生物学中受到了将它们输送到细胞中的复杂性的阻碍。在本报告中，我们证明了化学合成的抗癌肽有助于将发光量子点 (QD) 快速传递到活细胞中的有效性。我们结合荧光成像显微镜、流式细胞术和特定的内吞作用抑制实验来探测不同细胞系对 QD 肽缀合物的吸收。我们始终发现，相当大一部分内化缀合物不与内体或细胞核共定位。
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