The endogenous electric field (EF) of skin wounds plays an important role in the biological processes that underlie wound healing. Treatments that modulate wound-EFs promote healing. However, the mechanism(s) that underlie this effect remain unclear. Agilent-based microarrays were used to determine the transcriptomes of the keratinocyte line HaCaT, normal human dermal fibroblasts, and the human dermal endothelial cell line HMEC-1 before and after high-voltage alternating current (AC)-EF (14,000 V, 90 Hz) treatment. The keratinocytes had the most genes whose transcription was altered by EF. They included the cytochrome P450 (CYP) genes CYP1A1 and CYP1B1, HMOX1, EREG, DUSP5, and SLC7A11 (all upregulated), and DOCK8, ABCC6, and CYP26A1 (all downregulated). As shown by transcriptional-network analysis, all three CYP genes played central roles in the EF-induced changes in keratinocyte transcriptome. To the best of our knowledge, this is the first study that demonstrates that CYP genes play a key role in the transcriptional responses of human keratinocytes to EF treatment. Further investigations into the effects of EF on wound healing, aging, and regenerative medicine are likely to yield promising results.

皮肤伤口的内源性电场（EF）在构成伤口愈合基础的生物过程中起着重要作用。调节伤口EF的治疗可促进愈合。但是，影响这种作用的机制尚不清楚。基于安捷伦的微阵列用于确定高压交流电（AC）-EF（14,000 V，90 Hz）前后的角质形成细胞系HaCaT，正常人真皮成纤维细胞和人真皮内皮细胞系HMEC-1的转录组） 治疗。角质形成细胞的转录被EF改变的基因最多。它们包括细胞色素P450（CYP）基因CYP1A1和CYP1B1，HMOX1，EREG，DUSP5和SLC7A11（均被上调），以及DOCK8，ABCC6和CYP26A1（均被下调）。如转录网络分析所示，这三个CYP基因在EF诱导的角质形成细胞转录组变化中起着核心作用。据我们所知，这是第一项证明CYP基因在人角质形成细胞对EF治疗的转录反应中起关键作用的研究。进一步研究EF对伤口愈合，衰老和再生医学的影响可能会产生可喜的结果。