Continuous monocropping often influences negatively the soil microbial community and leads to the occurrence of soil-borne diseases. In this study, a pre-cultivation soil management strategy, reductive soil disinfestation (RSD), involving amendment by the use of reed straw, bagasse, and rice straw, and creating anaerobic soil conditions, was used to regulate the microbial community in a soil infested by Fusarium wilt of lisianthus and make it suitable for plant cultivation. The results showed that RSD significantly decreased F. oxysporum population by 97.1%–99.1% and the incidence of lisianthus wilt disease to 3.0%–14.3% compared with that of the untreated soil. The lowest disease incidence was found in the soil treated with RSD where bagasse was incorporated. The replantation of the host plant differently stimulated the pathogen proliferations across the different soils. MiSeq sequencing and culture-dependent investigation showed that the RSD treatments established distinct microbial communities compared to that of the untreated soil. Furthermore, the relative abundances of representatives of the families Cytophagaceae, Chitinophagaceae, Chaetomiaceae, and an unclassified family within Sordariomycetes, as well as soil microbial activity and the proportions of antagonists were significantly and negatively correlated with the pathogen population increase. Overall, the RSD treatment contributed to the reassembly of the soil microbiome which contained more beneficial agents that successfully controlled the pathogen inoculum level and lisianthus Fusarium wilt disease.

连续种植通常会对土壤微生物群落产生负面影响，并导致土壤传播疾病的发生。在这项研究中，采用了一种耕前土壤管理策略，即减少土壤除草（RSD），其中涉及通过使用芦苇秸秆，蔗渣和稻草进行改良，并创造厌氧土壤条件，以调节土壤中的微生物群落。以桔梗枯萎病为害，适合植物栽培。结果表明，RSD显着降低了尖孢镰刀菌与未经处理的土壤相比，该地区的人口增长了97.1％–99.1％，而桔梗枯萎病的发生率达到了3.0％–14.3％。在掺入蔗渣的RSD处理过的土壤中，该病的发病率最低。寄主植物的重新种植刺激了病原体在不同土壤中的扩散。MiSeq测序和培养依赖性研究表明，与未处理土壤相比，RSD处理建立了独特的微生物群落。此外，丝藻科，角膜藻科，Chaetomiaceae和伞形霉菌内一个未分类家族的代表的相对丰度，以及土壤微生物活性和拮抗剂的比例与病原菌种群的增加呈显着负相关。全面的，