The type VI secretion system (T6SS) is a versatile molecular machinery widely distributed in Gram-negative bacteria. The activity of the T6SS is tightly regulated by various mechanisms, including quorum sensing (QS), iron concentration, and transcriptional regulators. Here we demonstrated that the stringent response regulator, RelA, contributes to bacterial resistance to multiple environmental stresses in Yersinia pseudotuberculosis. We also revealed that the stress resistance function of stringent response (SR) was partially mediated by the general stress response T6SS4 system. RelA positively regulates the expression of T6SS4 to combat various stresses in response to nutrition starvation collectively mediated by the RovM and RovA regulators. These findings revealed not only the important role of T6SS4 in SR induced stress resistance, but also a new pathway to regulate T6SS4 expression in response to starvation stress.

VI型分泌系统（T6SS）是一种用途广泛的分子机器，广泛分布于革兰氏阴性细菌中。T6SS的活性受到各种机制的严格调控，包括群体感应（QS），铁浓度和转录调控因子。在这里，我们证明了严格的反应调节，的RelA，有利于细菌的耐药性在多种环境压力假结核菌。我们还揭示了严格反应（SR）的抗应激功能部分由一般的应激反应T6SS4系统介导。RelA积极调节T6SS4的表达，以应对由RovM和RovA调节剂共同介导的营养饥饿引起的各种压力。这些发现不仅揭示了T6SS4在SR诱导的抗逆性中的重要作用，而且揭示了调节T6SS4表达以应对饥饿胁迫的新途径。