The complete excision of glioblastomas with maximal retention of surrounding normal tissues can have a positive effect on the survival status and quality of life of patients. Near-infrared fluorescence (NIRF) optical imaging of the tumor vasculature offers a noninvasive method for detection of early stage glioblastoma and efficient monitoring of therapeutic responses. The aim of this study was to develop a novel NIRF imaging probe as a visualization tool for image-guided surgical resection of orthotopic glioblastoma. In this study, Cy5.5-RKL, Cy5.5-NKL, and Cy5.5-DKL probes were successfully synthesized, and their properties were investigated in vitro and in vivo. In vivo, Cy5.5-RKL and Cy5.5-NKL were able to detect U87MG xenografts for at least 8 h post injection. The maximum tumor-to-muscle ratios of Cy5.5-RKL and Cy5.5-NKL were 7.65 ± 0.72 and 5.43 ± 0.72, respectively. Of the probes, Cy5.5-RKL displayed the best delineation of the boundaries between orthotopic glioblastomas and normal brain tissue at 8 h p.i. In conclusion, NIRF imaging using Cy5.5-RKL is promising not only for diagnostic purposes but also for use in image-guided surgery for orthotopic glioblastoma or other superficial tumors.

中文翻译：

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具有高肿瘤选择性的近红外荧光肽：原位脑胶质瘤的图像引导手术切除的新型探针。

胶质母细胞瘤的完整切除和周围正常组织的最大保留可以对患者的生存状况和生活质量产生积极影响。肿瘤脉管系统的近红外荧光（NIRF）光学成像为检测早期胶质母细胞瘤和有效监测治疗反应提供了一种非侵入性方法。这项研究的目的是开发一种新型的NIRF成像探针，作为用于原位成胶质细胞瘤的图像引导手术切除的可视化工具。在这项研究中，成功​​地合成了Cy5.5-RKL，Cy5.5-NKL和Cy5.5-DKL探针，并在体内和体外研究了它们的特性。在体内，Cy5.5-RKL和Cy5.5-NKL能够在注射后至少8小时内检测到U87MG异种移植物。Cy5.5-RKL和Cy5.5-NKL的最大瘤肌比为7.65±0。分别为72和5.43±0.72。在探针中，Cy5.5-RKL在pi时8 h表现出原位胶质母细胞瘤与正常脑组织之间边界的最佳描绘。总之，使用Cy5.5-RKL进行NIRF成像不仅有望用于诊断目的，而且还可用于原位胶质母细胞瘤或其他浅表性肿瘤的图像引导手术。