当前位置: X-MOL 学术Aquat. Toxicol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Immunotoxic effects of 4-nonylphenol on Clarias gariepinus: Cytopathological changes in hepatic melanomacrophages
Aquatic Toxicology ( IF 4.1 ) Pub Date : 2018-12-03 , DOI: 10.1016/j.aquatox.2018.12.002
Alaa El-Din H. Sayed , Mahmoud Abd-Elkareem , Nasser S. Abou Khalil

Melanomacrophage centres (MMCs) play a key role in the immune response in fish. They are considered sensitive bio-monitoring structures with roles in the assessment of toxicant impacts. The aim of this study was to examine the potential histopathological effect of 4-nonylphenol (4-NP) on hepatic MMCs in Clarias gariepinus. To achieve this objective, adult male fish were divided randomly and equally into two groups: a control group and a group that was exposed to 4-NP (dissolved in water at a dose of 0.1 mg/L) for 21 days. The 4-NP-intoxicated hepatic MMCs contained numerous necrotic macrophages. Superoxide dismutase 2 was immuno-expressed in the hepatic MMCs in both groups, with no significant difference. Histomorphometric examination revealed that the sizes and numbers of MMCs were dramatically higher in the livers of 4-NP-exposed C. gariepinus than in control fish. Following 4-NP challenge, in the liver, the abundance of lipofuscin and haemosiderin pigments increased, and single-pigmented macrophages, aggregated groups of deformed red blood cells (RBCs) and macrophages were present near blood vessels and hepatic sinusoids. These results reveal that 4-NP exerts immunological effects on hepatic MMCs in C. gariepinus and support the utility of MMCs as a cytological biomarker for aquatic exposure to 4-NP.



中文翻译:

4-壬基酚对加里氏疟原虫的免疫毒性作用:肝黑色素巨噬细胞的细胞病理学变化

黑色素巨噬细胞中心(MMCs)在鱼类的免疫反应中起关键作用。它们被认为是敏感的生物监测结构,在评估有毒物质的影响中起作用。这项研究的目的是检查4-壬基酚(4-NP)对加里氏鞭毛虫肝MMC的潜在组织病理学影响。。为了实现这一目标,将成年雄鱼随机分为两组:对照组和暴露于4-NP(以0.1 mg / L的剂量溶于水中)达21天的一组。4-NP中毒的肝MMC包含大量坏死性巨噬细胞。两组肝MMC中均超表达超氧化物歧化酶2,差异无统计学意义。组织形态计量学检查显示,在暴露于4-NP的C. gariepinus肝脏中,MMC的大小和数量显着增加而不是对照鱼。4-NP攻击后,肝脏中的脂褐素和血红素色素丰富,并且在血管和肝正弦曲线附近出现了单色素巨噬细胞,变形红细胞(RBC)和巨噬细胞的聚集群。这些结果表明4-NP对加里螺旋藻中的肝MMC发挥免疫学作用,并支持MMC作为水生暴露于4-NP的细胞生物学标志物的效用。

更新日期:2018-12-03
down
wechat
bug