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The ameliorative effect of a Lactobacillus strain with good adhesion ability against dextran sulfate sodium-induced murine colitis†
Food & Function ( IF 5.1 ) Pub Date : 2018-12-03 00:00:00 , DOI: 10.1039/c8fo01453a Guangqiang Wang 1, 2, 3, 4, 5 , Yingnan Liu 1, 2, 3, 4, 5 , Zhi Lu 5, 6, 7 , Yiting Yang 5, 6, 7 , Yongjun Xia 1, 2, 3, 4, 5 , Phoency F.-H. Lai 1, 2, 3, 4, 5 , Lianzhong Ai 1, 2, 3, 4, 5
Food & Function ( IF 5.1 ) Pub Date : 2018-12-03 00:00:00 , DOI: 10.1039/c8fo01453a Guangqiang Wang 1, 2, 3, 4, 5 , Yingnan Liu 1, 2, 3, 4, 5 , Zhi Lu 5, 6, 7 , Yiting Yang 5, 6, 7 , Yongjun Xia 1, 2, 3, 4, 5 , Phoency F.-H. Lai 1, 2, 3, 4, 5 , Lianzhong Ai 1, 2, 3, 4, 5
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The objective of this study was to effectively screen out a Lactobacillus strain with excellent adhesion ability and ameliorative effect on the disease symptoms of a murine ulcerative colitis model. The auto-aggregation rate (RAA), co-aggregation rate with Escherichia coli O157 (RCA), and cell surface hydrophobicity (AHC) of 17 Lactobacillus strains were measured for primary selection. The results indicate that Lactobacillus plantarum AR326 displayed the topmost overall aggregation performance among all strains examined. A positive and linear relationship between RCA and RAA values was observed for 15 Lactobacillus strains with RAA = 16–46% and RCA = 15–27%. For adhesion ability to human adenocarcinoma HT-29 cells, five representative Lactobacillus strains showed a good, positive dependence on RAA, where L. plantarum AR326 and L. fermentum AR184 showed a higher adhesion ability than the others. Using the 5 (6)-carboxyfluorescein diacetate N-succinimidyl ester (cFDA SE)-labeling technique, L. plantarum AR326 was confirmed to adhere to and colonize well in the intestinal mucosa of mice mainly in the ileum and colon. Finally, L. plantarum AR326 at the dosage applied (daily 2 × 109 cfu per mouse) could attenuate murine dextran sulfate sodium (DSS)-induced colitis by effectively decreasing the body weight loss, disease activity index, colon length shortening, myeloperoxidase activity, and colon epithelial damage of experimental animals. The protective effects involved the restoration of the tight junction protein expression and reduction of the abnormal expression of pro-inflammatory cytokines. In conclusion, L. plantarum AR326 can be used as promising probiotics to ameliorate DSS-induced colitis.
中文翻译：
具有良好粘附能力 的乳酸杆菌菌株对硫酸葡聚糖钠诱导的鼠结肠炎的改善作用†
这项研究的目的是有效筛选出具有优异的粘附能力和对小鼠溃疡性结肠炎模型的疾病症状具有改善作用的乳杆菌菌株。为初步选择，对17株乳酸杆菌的自动聚集率（R AA），与大肠杆菌O157的共聚集率（R CA）和细胞表面疏水性（A HC）进行了测量。结果表明，植物乳杆菌AR326在所有检测的菌株中表现出最高的总体聚集性能。之间的正和线性关系ř CA和观察到15株乳酸菌的R AA值，其中R AA = 16–46％，R CA = 15–27％。用于粘附能力人腺癌HT-29细胞中，五个有代表性的乳杆菌属菌株显示出良好的，对正依赖性ř AA，其中植物乳杆菌AR326和发酵乳杆菌AR184显示出比其它更高的粘附能力。使用5（6）-羧基荧光素二乙酸酯N-琥珀酰亚胺酯（cFDA SE）标记技术，植物乳杆菌证实AR326粘附并很好地定居在小鼠的肠粘膜中，主要在回肠和结肠中。最后，应用剂量（每只小鼠每天2×10 9 cfu）的植物乳杆菌AR326可通过有效减少体重减轻，疾病活动指数，结肠长度缩短，髓过氧化物酶活性来减轻鼠用硫酸葡聚糖硫酸钠（DSS）引起的结肠炎。以及实验动物的结肠上皮损伤。保护作用涉及恢复紧密连接蛋白表达和减少促炎细胞因子的异常表达。总之，植物乳杆菌AR326可用作改善DSS诱导的结肠炎的有前途的益生菌。
更新日期：2018-12-03
中文翻译：
具有良好粘附能力 的乳酸杆菌菌株对硫酸葡聚糖钠诱导的鼠结肠炎的改善作用†
这项研究的目的是有效筛选出具有优异的粘附能力和对小鼠溃疡性结肠炎模型的疾病症状具有改善作用的乳杆菌菌株。为初步选择，对17株乳酸杆菌的自动聚集率（R AA），与大肠杆菌O157的共聚集率（R CA）和细胞表面疏水性（A HC）进行了测量。结果表明，植物乳杆菌AR326在所有检测的菌株中表现出最高的总体聚集性能。之间的正和线性关系ř CA和观察到15株乳酸菌的R AA值，其中R AA = 16–46％，R CA = 15–27％。用于粘附能力人腺癌HT-29细胞中，五个有代表性的乳杆菌属菌株显示出良好的，对正依赖性ř AA，其中植物乳杆菌AR326和发酵乳杆菌AR184显示出比其它更高的粘附能力。使用5（6）-羧基荧光素二乙酸酯N-琥珀酰亚胺酯（cFDA SE）标记技术，植物乳杆菌证实AR326粘附并很好地定居在小鼠的肠粘膜中，主要在回肠和结肠中。最后，应用剂量（每只小鼠每天2×10 9 cfu）的植物乳杆菌AR326可通过有效减少体重减轻，疾病活动指数，结肠长度缩短，髓过氧化物酶活性来减轻鼠用硫酸葡聚糖硫酸钠（DSS）引起的结肠炎。以及实验动物的结肠上皮损伤。保护作用涉及恢复紧密连接蛋白表达和减少促炎细胞因子的异常表达。总之，植物乳杆菌AR326可用作改善DSS诱导的结肠炎的有前途的益生菌。
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