In this study, the cytotoxicity of CdTe quantum dots (QDs) of various dimensions was examined using the electroporation method. The influence of the size of QDs on normal and tumour cell viability after 24 h of incubation with nanomaterials was examined. The three human cell lines were chosen for the tests: A549 (a tumour cell line derived from the lung), MRC-5 (normal fibroblasts from the lung) and HaCaT (normal keratinocytes from the skin). Accordingly, we modelled the effect of nanocrystals on various human tissues because nanoparticles can be introduced into an organism through different routes. We were also able to study which cells are more sensitive to nanoparticles: normal or tumour cells. The nanoparticles were introduced into cells through pores in the cell membranes that were generated by electrical pulses. The effectiveness of introducing nanocrystals into cells was determined as a function of the nanocrystal dimensions and accumulation locations. Moreover, the cytotoxicity of quantum dots was tested, and cell viability after electroporation was evaluated. We also investigated whether the introduced nanocrystals released cadmium ions.

在这项研究中，使用电穿孔方法检查了各种尺寸的CdTe量子点（QDs）的细胞毒性。与纳米材料孵育24小时后，检查了QD大小对正常细胞和肿瘤细胞生存力的影响。选择了三种人类细胞系进行测试：A549（源自肺的肿瘤细胞系），MRC-5（源自肺的正常成纤维细胞）和HaCaT（源自皮肤的正常角质形成细胞）。因此，我们对纳米晶体对各种人体组织的影响进行了建模，因为纳米颗粒可以通过不同的途径引入生物体。我们还能够研究哪些细胞对纳米颗粒更敏感：正常细胞还是肿瘤细胞。通过由电脉冲产生的细胞膜中的孔将纳米颗粒引入细胞。将纳米晶体引入细胞的效率取决于纳米晶体的尺寸和堆积位置。此外，测试了量子点的细胞毒性，并评估了电穿孔后的细胞活力。我们还研究了引入的纳米晶体是否释放了镉离子。