We thank Dr Pacheco and colleagues for their comments1 on our article2 on the variation of testing for antinuclear antibodies (ANA) by indirect immunofluorescence (IIF) in the context of systemic lupus erythematosus (SLE). Along with findings described in other letters,3–8 the observations by Pacheco and colleagues on three different line immunoassays (LIA) and an ELISA indicate that, as in the case of other technologies, LIAs can show considerable discordance among different kits that are currently available. This study also shows that results by LIA can differ from those obtained by an ELISA. Differences among ANA tests can have a variety of causes including assay conditions as well as the origin and biochemical properties of test antigens. …

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回应：Pacheco 等人的“抗核自身抗体：四种不同检测之间的不一致”

我们感谢 Pacheco 博士及其同事对我们关于在系统性红斑狼疮 (SLE) 背景下通过间接免疫荧光 (IIF) 检测抗核抗体 (ANA) 的变化的文章 2 的评论1。连同其他信件中描述的发现，3-8 Pacheco 及其同事对三种不同的线性免疫分析 (LIA) 和 ELISA 的观察表明，与其他技术一样，LIA 可以在当前使用的不同试剂盒之间表现出相当大的不一致。可用的。该研究还表明，LIA 的结果可能与 ELISA 获得的结果不同。ANA 测试之间的差异可能有多种原因，包括检测条件以及测试抗原的来源和生化特性。…