Hypertension is a civilization disease leading to remodeling and damage of blood vessels, impaired renal function and premature death. The aim of this study was to compare the effect of chronic administration of URB597, the FAAH (fatty acid amide hydrolase) inhibitor, to rats with primary (SHRs) and secondary (DOCA-salt hypertensive rats) hypertension on electrical and physicochemical properties of kidney cells membranes. Changes in the electrical charge of the membrane may affect the cell functions. The electrical properties of the kidney cells (surface charge density, zeta potential) were measured by electrophoresis. Qualitative and quantitative composition of the membrane (phospholipids and proteins) was determined by HPLC and lipid peroxidation product (4-hydroxy-2E-hexenal; 4-HHE) level was examined by GCMSMS, while the sialic acid content was measured by resorcinol method. In rats with primary hypertension (SHR) and secondary hypertension (DOCA-salt), changes in electrical properties (increase of electric charge and zeta potential) and membrane composition (increase in sialic acid and protein concentration and decrease in phospholipid level) of kidney cells are observed in comparison to control animals. Greater changes were observed in DOCA-salt hypertensive rats. Changes in membrane properties caused by URB597 depend on the type of hypertension. The administration of URB597 to rats with primary hypertension partially prevents changes in the electrical properties (electrical charge, zeta potential) of the membrane caused by hypertension as well as in the sialic acid and proteins content. However, there is no reduction in oxidative stress, assessed by the level of 4-HHE, which may affect the metabolic function of the kidneys. URB597 administered to rats with DOCA salt does not prevent, but rather intensifies, changes caused by hypertension in the kidney. In conclusion, URB597 given to individuals with hypertension, particularly with secondary hypertension, enhancing some disturbances in electric and physicochemical properties of kidney cells observed in hypertension what may lead to additional kidney disorders. Therefore, further researches are necessary.

高血压是一种文明疾病，会导致血管重塑和损坏，肾功能受损和过早死亡。这项研究的目的是比较长期给予原发性（SHRs）和继发性（DOCA-盐高血压大鼠）高血压大鼠FAAH（脂肪酸酰胺水解酶）抑制剂URB597对肾脏电和理化性质的影响细胞膜。膜电荷的变化可能会影响细胞功能。通过电泳测量肾细胞的电特性（表面电荷密度，ζ电势）。通过HPLC测定膜的定性和定量组成（磷脂和蛋白质），通过GCMSMS检查脂质过氧化产物（4-羟基-2E-己烯醛； 4-HHE）的水平，唾液酸含量用间苯二酚法测定。在患有原发性高血压（SHR）和继发性高血压（DOCA-盐）的大鼠中，肾细胞的电特性（电荷和zeta电位增加）和膜组成（唾液酸和蛋白质浓度增加并且磷脂水平降低）变化与对照动物相比，观察到了“”。在DOCA-盐高血压大鼠中观察到更大的变化。由URB597引起的膜特性变化取决于高血压的类型。向患有原发性高血压的大鼠施用URB597可以部分预防由高血压引起的膜的电特性（电荷，ζ电位）的变化以及唾液酸和蛋白质含量的变化。但是，氧化应激并没有降低，通过4-HHE的水平评估，可能会影响肾脏的代谢功能。用DOCA盐对大鼠施用URB597不能预防但会加剧由肾脏高血压引起的变化。总之，URB597适用于患有高血压的人，特别是继发性高血压的人，增强了高血压中观察到的肾细胞的电和理化性质的某些紊乱，这可能导致其他肾脏疾病。因此，有必要做进一步的研究。增强高血压中观察到的肾细胞的电和理化特性的某些紊乱，这可能导致其他肾脏疾病。因此，有必要做进一步的研究。增强高血压中观察到的肾细胞的电和理化特性的某些紊乱，这可能导致其他肾脏疾病。因此，有必要做进一步的研究。