Pollen grains are tiny structures vital for sexual reproduction and consequently seed and fruit production in angiosperms, and a source of many allergenic components responsible for deleterious implications for health worldwide. Current pollen research is mainly focused on unraveling the molecular mechanisms underlying the pollen germination and tube formation passing from the quiescent stage. In this context, an in‐depth proteome analysis of the pollens from Ricinus communis at three different stages—that is, mature, hydrated, and in vitro germinated—is performed. This analysis results in the identification of 1950 proteins, including 1773, 1313, and 858, from mature, hydrated, and germinated pollens, respectively. Based on label‐free quantification, 164 proteins are found to be significantly differentially abundant from mature to hydrated pollens, 40 proteins from hydrated to germinated, and 57 proteins from mature to germinated pollens, respectively. Most of the differentially abundant proteins are related to protein, carbohydrate, and energy metabolism and signaling. Besides other functional classes, a reasonable number of the proteins are predicted to be allergenic proteins, previously undiscovered. This is the first in‐deep proteome analysis of the R. communis pollens and, to the best of our knowledge, one of the most complete proteome dataset identified from the pollens of any plant species, thus providing a reference proteome for researchers interested in pollen biology.

中文翻译：

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蓖麻花粉的深度蛋白质组分析

花粉粒是微小的结构，对于被子植物的有性繁殖以及种子和果实的生产至关重要，并且是许多致敏成分的来源，这些成分对全世界的健康造成有害影响。当前的花粉研究主要集中于揭示从静止期开始的花粉萌发和管形成的分子机制。在这种情况下，对蓖麻（Ricinus communis）的花粉进行了深入的蛋白质组分析在三个不同的阶段-成熟，水合和体外发芽-进行。该分析可鉴定出分别来自成熟，水合和发芽花粉的1950种蛋白质，包括1773、1313和858。根据无标记的定量分析，发现从成熟花粉到水合花粉，分别有164种蛋白质差异显着；从水化花粉到发芽的花粉分别有40种蛋白质，从成熟到发芽的花粉分别有57种蛋白质。大多数差异丰富的蛋白质都与蛋白质，碳水化合物以及能量代谢和信号传导有关。除其他功能类别外，预计有相当数量的蛋白质是以前未发现的变应原性蛋白质。这是对R. communis的首次深入蛋白质组分析 花粉，据我们所知，是从任何植物物种的花粉中鉴定出的最完整的蛋白质组数据集之一，从而为对花粉生物学感兴趣的研究人员提供了参考蛋白质组。