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Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation.
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2018-11-20 , DOI: 10.1111/1751-7915.13335 Xiaoshu Jing 1 , Qingwen Cui 1 , Xiaochen Li 1 , Jia Yin 1 , Vinothkannan Ravichandran 1 , Deng Pan 2 , Jun Fu 1 , Qiang Tu 1 , Hailong Wang 1 , Xiaoying Bian 1 , Youming Zhang 1
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2018-11-20 , DOI: 10.1111/1751-7915.13335 Xiaoshu Jing 1 , Qingwen Cui 1 , Xiaochen Li 1 , Jia Yin 1 , Vinothkannan Ravichandran 1 , Deng Pan 2 , Jun Fu 1 , Qiang Tu 1 , Hailong Wang 1 , Xiaoying Bian 1 , Youming Zhang 1
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In agricultural production, sustainability is currently one of the most significant concerns. The genetic modification of plant growth‐promoting rhizobacteria may provide a novel way to use natural bacteria as microbial inoculants. In this study, the root‐colonizing strain Pseudomonas protegens Pf‐5 was genetically modified to act as a biocontrol agent and biofertilizer with biological nitrogen fixation activity. Genetic inactivation of retS enhanced the production of 2,4‐diacetylphloroglucinol, which contributed for the enhanced antifungal activity. Then, the entire nitrogenase island with native promoter from Pseudomonas stutzeri DSM4166 was introduced into a retS mutant strain for expression. Root colonization patterns assessed via confocal laser scanning microscopy confirmed that GFP‐tagged bacterial were mainly located on root surfaces and at the junctions between epidermal root cells. Moreover, under pathogen and N‐limited double treatment conditions, the fresh weights of seedlings inoculated with the recombinant retS mutant‐nif strain were increased compared with those of the control. In conclusion, this study has innovatively developed an eco‐friendly alternative to the agrochemicals that will benefit global plant production significantly.
中文翻译：
改造 Pseudomonas protegens Pf-5 以提高其抗真菌活性和固氮作用。
在农业生产中，可持续性是目前最重要的问题之一。促进植物生长的根际细菌的遗传修饰可能为利用天然细菌作为微生物接种剂提供一种新方法。在这项研究中，对根系定殖菌株Pseudomonas protegens Pf-5 进行了基因改造，以作为具有生物固氮活性的生物防治剂和生物肥料。retS的遗传失活增强了 2,4-二乙酰间苯三酚的产生，这有助于增强抗真菌活性。然后，将具有来自施氏假单胞菌DSM4166的天然启动子的整个固氮酶岛引入retS用于表达的突变株。通过共聚焦激光扫描显微镜评估的根定殖模式证实，GFP 标记的细菌主要位于根表面和表皮根细胞之间的连接处。此外，在病原菌和限氮双重处理条件下，与对照相比，接种重组retS突变体-nif菌株的幼苗鲜重增加。总之，本研究创新地开发了一种环保的农用化学品替代品，将显着造福全球植物生产。
更新日期：2018-11-20
中文翻译：
改造 Pseudomonas protegens Pf-5 以提高其抗真菌活性和固氮作用。
在农业生产中，可持续性是目前最重要的问题之一。促进植物生长的根际细菌的遗传修饰可能为利用天然细菌作为微生物接种剂提供一种新方法。在这项研究中，对根系定殖菌株Pseudomonas protegens Pf-5 进行了基因改造，以作为具有生物固氮活性的生物防治剂和生物肥料。retS的遗传失活增强了 2,4-二乙酰间苯三酚的产生，这有助于增强抗真菌活性。然后，将具有来自施氏假单胞菌DSM4166的天然启动子的整个固氮酶岛引入retS用于表达的突变株。通过共聚焦激光扫描显微镜评估的根定殖模式证实，GFP 标记的细菌主要位于根表面和表皮根细胞之间的连接处。此外，在病原菌和限氮双重处理条件下，与对照相比，接种重组retS突变体-nif菌株的幼苗鲜重增加。总之，本研究创新地开发了一种环保的农用化学品替代品，将显着造福全球植物生产。
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