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Antinuclear autoantibodies: discordance among four different assays
Annals of the Rheumatic Diseases ( IF 20.3 ) Pub Date : 2018-11-20 , DOI: 10.1136/annrheumdis-2018-214693
Yovana Pacheco 1 , Diana M Monsalve 1 , Yeny Acosta-Ampudia 1 , Cilia Rojas 1, 2 , Juan-Manuel Anaya 1 , Carolina Ramírez-Santana 3
Affiliation  

Detection of antinuclear antibodies (ANAs) is of paramount importance for diagnosis and assessment of autoimmune diseases (ADs), but may vary depending on the method used. Therefore, we aimed to compare three different line immunoassays (LIAs) for ANAs testing in order to simultaneously evaluate their concordance with the results obtained by a well known and widely used ELISA. Sera from women with established systemic lupus erythematosus (SLE, n=62) and Sjogren’s syndrome (SS, n=28) were examined. Patients with SLE had an average age of 48.7 years (IQR 36.8–57.0) with a disease duration of 14.8 years (IQR 9.0–18.0). Patients with SS had an average age of 62.1 years (IQR 55.5–71.0) with a duration of disease of 13.1 years (IQR 8.3–17). Table 1 shows the results from the two-by-two comparison for the four techniques. Different levels of agreement were observed among the assays. In general, they were deficient. The analysis showed only one ‘strong’ level of agreement between ELISA and IMTEC-ANA-LIA Maxx for anti-Ro measurement. Out of 39 comparisons, 14 did …

中文翻译:

抗核自身抗体:四种不同检测方法之间的不一致

抗核抗体 (ANA) 的检测对于自身免疫性疾病 (AD) 的诊断和评估至关重要,但可能因所使用的方法而异。因此,我们旨在比较用于 ANA 测试的三种不同的线性免疫测定 (LIA),以便同时评估它们与众所周知且广泛使用的 ELISA 获得的结果的一致性。检查了患有系统性红斑狼疮(SLE,n=62)和干燥综合征(SS,n=28)的女性的血清。SLE 患者的平均年龄为 48.7 岁(IQR 36.8-57.0),病程为 14.8 年(IQR 9.0-18.0)。SS 患者的平均年龄为 62.1 岁(IQR 55.5-71.0),病程为 13.1 年(IQR 8.3-17)。表 1 显示了对四种技术进行两两比较的结果。在测定中观察到不同程度的一致性。总的来说,他们是有缺陷的。分析表明 ELISA 和 IMTEC-ANA-LIA Maxx 在抗 Ro 测量方面只有一个“强”水平的一致性。在 39 次比较中,有 14 次做了……
更新日期:2018-11-20
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