Here, the confined electrochemiluminescence (ECL) of luminol and hydrogen peroxide is achieved in vertically ordered silica mesochannels (SMCs) and applied to map the efflux of hydrogen peroxide from single living cells. The vertical alignment of SMCs on indium tin oxide (ITO) slides restricts the lateral diffusion of species generated in the ECL process. Accordingly, the cross-talk of luminescence intensity from nearby microregions is minimized for higher-spatial-resolution ECL imaging. The characterization of luminol ECL on these SMC-coated slides yields a detection range of hydrogen peroxide between 5 μM and 1 mM, providing high sensitivity in imaging the efflux of hydrogen peroxide from cells. Compared with the luminescence from individual cells on bare ITO slides, ~4-fold intensity is observed for SMC-coated ITO, suggesting a better electrode surface for single-cell ECL imaging.

在这里，在垂直有序的二氧化硅介观通道（SMC）中实现了鲁米诺和过氧化氢的局限性电化学发光（ECL），并用于绘制单个活细胞中过氧化氢的流出。SMC在铟锡氧化物（ITO）滑片上的垂直排列限制了ECL工艺中产生的物质的横向扩散。因此，来自附近的微区的发光强度的串扰被最小化以用于更高空间分辨率的ECL成像。在这些涂有SMC的载玻片上对鲁米诺ECL进行表征可产生5μM至1 mM的过氧化氢检测范围，在成像过氧化氢从细胞中流出时提供了很高的灵敏度。与裸ITO载玻片上单个细胞的发光相比，SMC涂覆的ITO的发光强度约为4倍，