Here, an ultrasensitive and low-cost electrochemical aptasensing assay is developed based on the applicability of a fabricated nanocomposite from nitrogen-doped graphene quantum dots (N-GQDs) and gold nanoparticles (AuNPs). A modified glassy carbon electrode (GCE) with the [email protected] nanocomposite ([email protected]/GCE) as an efficient platform has some unique properties such as high surface area and electrical conductivity. Furthermore, the prepared platform is capable of more loading of aptamer (Apt) molecules as a biological recognition element of Ibuprofen (IBP) on the modified electrode surface. It is noteworthy that in this study, riboflavin (RF) as a universal green probe is used for the first time for electrochemical detection of IBP. According to the proposed strategy and under the optimum condition, the unprecedented detection limit (LOD) of this assay (33.33 aM) is lower than previously reported analytical methods. The results demonstrate the ability of the nanocomposite for designing of the aptasensor, integrated within the electrode format, to cheaper and simpler detection of the IBP with a specificity and sensitivity sufficient for analysis in real samples. It seems that the proposed strategy based on the [email protected] nanocomposite can be expanded to other nanomaterials. So, this is expected to have promising implications in the design of electrochemical sensors or biosensors for the detection of various targets.

在此，基于由掺杂氮的石墨烯量子点（N-GQDs）和金纳米颗粒（AuNPs）制成的纳米复合材料的适用性，开发了一种超灵敏，低成本的电化学适体测定法。以[电子邮件保护]纳米复合材料（[电子邮件保护] / GCE）为有效平台的修饰玻碳电极（GCE）具有一些独特的特性，例如高表面积和导电性。此外，所制备的平台能够在修饰的电极表面上更多地装载适体（Apt）分子作为布洛芬（IBP）的生物识别元件。值得注意的是，在这项研究中，核黄素（RF）作为通用的绿色探针首次用于IBP的电化学检测。根据拟议的策略并在最佳条件下，该检测方法（33.33 aM）的空前检测极限（LOD）低于以前报道的分析方法。结果表明，纳米复合材料能够以电极形式集成在适体传感器的设计中，从而能够更便宜，更简单地检测IBP，其特异性和灵敏度足以在真实样品中进行分析。似乎基于[受电子邮件保护的]纳米复合材料的拟议策略可以扩展到其他纳米材料。因此，预期这将对用于检测各种目标的电化学传感器或生物传感器的设计产生有希望的影响。以更便宜，更简单的方式对IBP进行检测，其特异性和灵敏度足以在真实样品中进行分析。似乎基于[受电子邮件保护的]纳米复合材料的拟议策略可以扩展到其他纳米材料。因此，预期这将对用于检测各种目标的电化学传感器或生物传感器的设计产生有希望的影响。以更便宜，更简单的方式对IBP进行检测，其特异性和灵敏度足以在真实样品中进行分析。似乎基于[受电子邮件保护的]纳米复合材料的拟议策略可以扩展到其他纳米材料。因此，预期这将对用于检测各种目标的电化学传感器或生物传感器的设计产生有希望的影响。