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Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour
npj Precision Oncology ( IF 6.8 ) Pub Date : 2018-11-19 , DOI: 10.1038/s41698-018-0069-7
N. Malara , F. Gentile , N. Coppedè , M. L. Coluccio , P. Candeloro , G. Perozziello , L. Ferrara , M. Giannetto , M. Careri , A. Castellini , C. Mignogna , I. Presta , C. K. Pirrone , D. Maisano , A. Donato , G. Donato , M. Greco , D. Scumaci , G. Cuda , F. Casale , E. Ferraro , S. Bonacci , V. Trunzo , V. Mollace , V. Onesto , R. Majewska , F. Amato , M. Renne , N. Innaro , G. Sena , R. Sacco , F. Givigliano , C. Voci , G. Volpentesta , G. Guzzi , A. Lavano , E. Scali , U. Bottoni , E. Di Fabrizio

Secretome of primary cultures is an accessible source of biological markers compared to more complex and less decipherable mixtures such as serum or plasma. The protonation state (PS) of secretome reflects the metabolism of cells and can be used for cancer early detection. Here, we demonstrate a superhydrophobic organic electrochemical device that measures PS in a drop of secretome derived from liquid biopsies. Using data from the sensor and principal component analysis (PCA), we developed algorithms able to efficiently discriminate tumour patients from non-tumour patients. We then validated the results using mass spectrometry and biochemical analysis of samples. For the 36 patients across three independent cohorts, the method identified tumour patients with high sensitivity and identification as high as 100% (no false positives) with declared subjects at-risk, for sporadic cancer onset, by intermediate values of PS. This assay could impact on cancer risk management, individual’s diagnosis and/or help clarify risk in healthy populations.



中文翻译:

超疏水芯片实验室可测量分泌物组质子化状态并提供散发性肿瘤的个性化风险评估

与更复杂,更难以辨认的混合物(如血清或血浆)相比,原代培养物的分泌组是生物标志物的可及来源。分泌组的质子化状态(PS)反映细胞的代谢,可用于癌症的早期检测。在这里,我们演示了一种超疏水性有机电化学装置,该装置可测量源自液体活检组织的一滴分泌蛋白质组中的PS。利用来自传感器的数据和主成分分析(PCA),我们开发了能够有效地区分肿瘤患者和非肿瘤患者的算法。然后,我们使用质谱和样品的生化分析验证了结果。对于三个独立队列中的36名患者,该方法鉴定出具有高敏感性的肿瘤患者,并通过PS的中间值将散发性癌症发作的被宣布为高风险的受试者确定为100%(无假阳性)。该测定法可能会影响癌症风险管理,个人诊断和/或帮助阐明健康人群中的风险。

更新日期:2018-11-19
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