Post-transcriptional RNA metabolic pathways play important roles in permitting prokaryotes to operate under a variety of environmental conditions. Although significant progress has been made during the last decade in deciphering RNA processing pathways in a number of bacteria, a complete understanding of post-transcriptional RNA metabolism in any single microorganism is far from reality. Here we describe multiple experimental approaches that can be used to study mRNA stability, tRNA and rRNA processing, sRNA metabolism, and polyadenylation in prokaryotes. The methods described here can be readily utilized in both Gram-negative and Gram-positive bacteria with simple modifications.

中文翻译：

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原核生物转录后RNA代谢分析


转录后 RNA 代谢途径在原核生物在各种环境条件下发挥重要作用。尽管过去十年在破译许多细菌的 RNA 加工途径方面取得了重大进展，但对任何单一微生物的转录后 RNA 代谢的全面了解还远未实现。在这里，我们描述了可用于研究原核生物中 mRNA 稳定性、tRNA 和 rRNA 加工、sRNA 代谢和多聚腺苷酸化的多种实验方法。这里描述的方法可以通过简单的修改轻松用于革兰氏阴性和革兰氏阳性细菌。