Fluorescent Tracking of the Endoplasmic Reticulum in Live Pathogenic Fungal Cells,ACS Chemical Biology

当前位置： X-MOL 学术 › ACS Chem. Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Fluorescent Tracking of the Endoplasmic Reticulum in Live Pathogenic Fungal Cells
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2018-11-14 00:00:00 , DOI: 10.1021/acschembio.8b00782
Raphael I. Benhamou ₁ , Qais Z. Jaber ₁ , Ido M. Herzog ₁ , Yael Roichman ₁ , Micha Fridman ₁

Affiliation

In fungal cells, the endoplasmic reticulum (ER) harbors several of the enzymes involved in the biosynthesis of ergosterol, an essential membrane component, making this organelle the site of action of antifungal azole drugs, used as a first-line treatment for fungal infections. This highlights the need for specific fluorescent labeling of this organelle in cells of pathogenic fungi. Here we report on the development and evaluation of a collection of fluorescent ER trackers in a panel of Candida, considered the most frequently encountered pathogen in fungal infections. These trackers enabled imaging of the ER in live fungal cells. Organelle specificity was associated with the expression of the target enzyme of antifungal azoles that resides in the ER; specific ER labeling was not observed in mutant cells lacking this enzyme. Labeling of live Candida cells with a combination of a mitotracker and one of the novel fungal ER trackers revealed sites of contact between the ER and mitochondria. These fungal ER trackers therefore offer unique molecular tools for the study of the ER and its interactions with other organelles in live cells of pathogenic fungi.

中文翻译：

活病原性真菌细胞中内质网的荧光跟踪

在真菌细胞中，内质网（ER）含有多种与麦角固醇（一种重要的膜成分）的生物合成有关的酶，使该细胞器成为抗真菌唑类药物的作用部位，被用作真菌感染的一线治疗方法。这突显了在病原性真菌细胞中需要对该细胞器进行特异性荧光标记。在这里，我们在念珠菌研究小组中报告了一系列荧光ER示踪剂的开发和评估，被认为是真菌感染中最常遇到的病原体。这些跟踪器使活真菌细胞中的ER成像成为可能。细胞器的特异性与驻留在ER中的抗真菌唑类目标酶的表达有关。在缺乏这种酶的突变细胞中未观察到特异性的ER标记。结合线粒体示踪剂和一种新型真菌ER示踪剂对活念珠菌细胞进行标记，揭示了ER和线粒体之间的接触位点。因此，这些真菌ER追踪器为研究ER及其与病原性真菌活细胞中其他细胞器的相互作用提供了独特的分子工具。

更新日期：2018-11-14

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11