The growth plate is a cartilaginous layer present from the gestation period until the end of puberty where it ossifies joining diaphysis and epiphysis. During this period several endocrine, autocrine, and paracrine processes within the growth plate are carried out by chondrocytes; therefore, a disruption in cellular functions may lead to pathologies affecting bone development. It is known that electric fields impact the growth plate; however, parameters such as stimulation time and electric field intensity are not well documented. Accordingly, this study presents a histomorphometrical framework to assess the effect of electric fields on chondroepiphysis explants. Bones were stimulated with 3.5 and 7 mV/cm, and for each electric field two exposure times were tested for 30 days (30 min and 1 h). Results evidenced that electric fields increased the hypertrophic zones compared with controls. In addition, a stimulation of 3.5 mV/cm applied for 1 h preserved the columnar cell density and its orientation. Moreover, a pre-hypertrophy differentiation in the center of the chondroepiphysis was observed when explants were stimulated during 1 h with both electric fields. These findings allow the understanding of the effect of electrical stimulation over growth plate organization and how the stimulation modifies chondrocytes morphophysiology.

生长板是从妊娠期一直到青春期末的软骨层，在该处骨化骨干和骨epi。在此期间，软骨细胞在生长平板内进行了几种内分泌，自分泌和旁分泌过程。因此，细胞功能的破坏可能导致影响骨骼发育的病理。众所周知，电场会影响生长板。然而，诸如刺激时间和电场强度之类的参数并没有得到很好的记录。因此，这项研究提出了一个组织形态计量学框架，以评估电场对软骨表physi外植体的影响。用3.5和7 mV / cm刺激骨骼，对于每个电场，测试两次暴露时间30天（30分钟和1小时）。结果证明，与对照组相比，电场增加了肥大区。此外，施加3.5 mV / cm的刺激1 h可以保持柱状细胞密度及其方向。此外，当两个电场在1 h内刺激外植体时，软骨软骨中央的肥大前分化也被观察到。这些发现可以理解电刺激对生长板组织的影响，以及刺激如何改变软骨细胞的形态生理。当两个电场在1 h内刺激外植体时，可以观察到软骨骨center中央肥大前的分化。这些发现可以理解电刺激对生长板组织的影响，以及刺激如何改变软骨细胞的形态生理。当两个电场在1 h内刺激外植体时，可以观察到软骨骨center中央肥大前的分化。这些发现可以理解电刺激对生长板组织的影响，以及刺激如何改变软骨细胞的形态生理。