A collaborative work on quantitative HPLC methods for the routine determination of atranol and chloroatranol in moss extracts,Flavour and Fragrance Journal

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A collaborative work on quantitative HPLC methods for the routine determination of atranol and chloroatranol in moss extracts
Flavour and Fragrance Journal ( IF 2.1 ) Pub Date : 2018-11-04 , DOI: 10.1002/ffj.3475
Jean-François Goursot ₁ ,

Affiliation

Moss extracts have been found to initiate skin sensitization. For this reason, oakmoss extracts are part of the diagnostic screening test for fragrance contact allergy. Atranol and chloroatranol are suspected to be responsible for the sensitizing properties of these extracts, and their content has consequently been limited to 100 ppm in moss extracts by the International Fragrance Association (IFRA). In this context, using round‐robin tests, PRODAROM (the French national association of fragrance manufacturers) has developed different analytical methods for the routine quantification of these two suspected skin allergens in moss extracts that are easily applicable in quality control laboratories. Reversed‐phase high‐performance liquid chromatography (HPLC) with diode‐array detection (DAD) and external calibration allowed the quantification of both analytes in solution in the 10–100 mg L⁻¹ range. In order to enhance the specificity of the method, mass spectrometry (MS) in single ion monitoring (SIM) mode was also used. To solve matrix‐effect problems, the use of in‐matrix calibration solutions, or the extrapolative dilution approach (EDA) using solvent calibration were mandatory. The liquid chromatography–mass spectrometry (LC‐MS) method was validated in the 1–10 mg L⁻¹ range for both allergens in solution.

中文翻译：

定量HPLC方法的合作研究，用于常规测定苔藓提取物中的阿特拉诺和氯阿特拉诺

已发现苔藓提取物可引起皮肤过敏。因此，橡苔提取物是对香精接触过敏的诊断筛选测试的一部分。怀疑阿特拉诺尔和氯三醇是这些提取物的致敏特性，因此，国际香料协会（IFRA）将青苔提取物中的它们含量限制为100 ppm。在这种情况下，PRODAROM（法国香料制造商全国协会）使用循环法测试，开发了不同的分析方法，用于对苔藓提取物中的这两种可疑皮肤过敏原进行常规定量分析，这些方法很容易应用于质量控制实验室。^-1范围。为了提高方法的特异性，还使用了单离子监测（SIM）模式下的质谱（MS）。为了解决基质效应问题，必须使用基质内校准溶液或使用溶剂校准的外推稀释法（EDA）。液相色谱-质谱法（LC-MS）在溶液中的两种过敏原均在1-10 mg L ^-1范围内得到验证。

更新日期：2018-11-04

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