Dioxin-like compounds (DLCs) are extremely stable toxic organic compounds and can cause serious health risks. To develop a convenient biomonitoring tool for the detection of DLCs in the environment, we generated a transgenic line—Tg(cyp1a-12DRE:EGFP)—with a zebrafish cyp1a promoter recombined with multiple dioxin-responsive elements (DREs) that drive EGFP expression. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)-induced EGFP expression was observed in the head cartilage (most sensitive), gut, otic vesicle, pectoral fin bud and eye of larvae. The lowest observed effect concentration of TCDD was estimated to be approximately 1 ng/L. Compared with existing zebrafish lines, our transgenic fish displayed comparable or even higher detection sensitivity to DLCs and could serve as an improved and rapid assay in an in vivo context. The Tg(cyp1a-12DRE:EGFP) transgenic zebrafish line also had higher stability for inducing EGFP expression (nearly 100% of our zebrafish induced EGFP at approximately 1 ng/L TCDD) than other lines. In addition, Tg(cyp1a-12DRE:EGFP) zebrafish could serve as a convenient and straightforward tool to assess potential cranial malformations and related health effects.

类二恶英化合物（DLC）是极其稳定的有毒有机化合物，可能会造成严重的健康风险。为了开发用于检测环境中DLC的便捷生物监控工具，我们生成了带有斑马鱼cy p1 a的转基因品系Tg（cy p1 a-12DRE：EGFP）。启动子与驱动EGFP表达的多种二恶英反应元件（DRE）重组。在头软骨（最敏感），肠，耳囊，胸鳍芽和幼虫的眼中观察到2,3,7,8-四氯二苯并-对二恶英（TCDD）诱导的EGFP表达。TCDD的最低观察到的效应浓度估计约为1 ng / L。与现有的斑马鱼品系相比，我们的转基因鱼显示出与DLC相当甚至更高的检测灵敏度，并且可以在体内进行改进和快速的检测。在TG（CY P1一个-12DRE：EGFP）转基因斑马鱼线也有用于诱导的EGFP表达（接近100％我们的斑马鱼的诱导EGFP在约1毫微克/ L TCDD）比其他线更高的稳定性。此外，Tg（cy p1 a-12DRE：EGFP）斑马鱼可以作为方便和直接的工具来评估潜在的颅骨畸形和相关的健康影响。