The aim of this study was to examine the effect of chromium(III), iron(III), molybdenum(III) and nickel(II) and their combinations on pro- and antioxidant activity in mouse embryo fibroblasts and liver cancer cells.

The present study shows that chromium(III), iron(III), nickel(II) and molybdenum(III) induce oxidative stress. In the case of chromium(III), nickel(II) and molybdenum(III) the intracellular ROS were dominant. However, in the case of iron(III) MDA was dominant - the end product of lipid peroxidation. Antioxidant activity of superoxide dismutase and catalase increased in low concentration of chromium(III); however, they decreased in higher concentrations. The same enzymes decreased after iron(III), nickel(II) and molybdenum(III) treatment in dose dependent manner. The activity of glutathione peroxidise decreased in dose dependent manner in all used microelements.

Additions of Cr(III) at 200 μM plus Fe(III) at 1000 μM showed synergistic effect in ROS production and in lowering antioxidant activity. The same type of interaction in pairs Cr(III) at 1000 μM plus Fe(III) or Ni(II) or Mo(III) at concentration of 200 μM was observed. The protective effects of Cr(III) in antioxidant activity and in lowering intracellular ROS production in pairs of Cr(III) at 200 μM and Ni(II) or Mo(III) at concentration of 1000 μM were observed.

这项研究的目的是检查铬（III），铁（III），钼（III）和镍（II）及其组合对小鼠胚胎成纤维细胞和肝癌细胞中前和抗氧化活性的影响。

本研究表明，铬（III），铁（III），镍（II）和钼（III）会引起氧化应激。在铬（III），镍（II）和钼（III）的情况下，细胞内ROS占优势。但是，在铁（III）的情况下，丙二醛占主导地位-脂质过氧化作用的终产物。低浓度铬（III）时超氧化物歧化酶和过氧化氢酶的抗氧化活性增强；然而，它们在较高浓度下下降。铁（III），镍（II）和钼（III）处理后，相同的酶以剂量依赖性方式降低。在所有使用的微量元素中，谷胱甘肽过氧化物的活性以剂量依赖性方式降低。

添加200μM的Cr（III）和1000μM的Fe（III）在ROS产生和降低抗氧化活性方面显示出协同作用。在浓度为200μM的1000μM的Cr（III）与Fe（III）或Ni（II）或Mo（III）的配对中观察到相同类型的相互作用。观察到Cr（III）在200μM的Cr（III）和1000μM的浓度的Ni（II）或Mo（III）对中的抗氧化活性和降低细胞内ROS生成的保护作用。