Class I terpene synthases generate the structural core of bioactive terpenoids. Deciphering structure–function relationships in the reactive closed complex and targeted engineering is hampered by highly dynamic carbocation rearrangements during catalysis. Available crystal structures, however, represent the open, catalytically inactive form or harbor nonproductive substrate analogs. Here, we present a...

中文翻译：

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识别控制I类萜烯合酶封闭复合物中催化作用的氨基酸网络[生物物理学和计算生物学]

I类萜烯合酶产生生物活性萜类化合物的结构核心。催化过程中高动态碳正离子重排阻碍了反应性封闭复合物和目标工程中结构与功能之间的解密关系。但是，可用的晶体结构代表开放的，催化惰性的形式或包含非生产性底物类似物。在这里，我们提出一个...