Solid‐state nanopores are an emerging biosensor for nucleic acid and protein characterization. For use in a clinical setting, solid‐state nanopore sensing requires sample preparation and purification, fluid handling, a heating element, electrical noise insulators, and an electrical readout detector, all of which hamper its translation to a point‐of‐care diagnostic device. A stand‐alone microfluidic‐based nanopore device is described that combines a bioassay reaction/purification chamber with a solid‐state nanopore sensor. The microfluidic device is composed of the high‐temperature/solvent resistance Zeonex plastic, formed via micromachining and heat bonding, enabling the use of both a heat regulator and a magnetic controller. Fluid control through the microfluidic channels and chambers is controlled via fluid port selector valves and allows up to eight different solutions. Electrical noise measurements and DNA translocation experiments demonstrate the integrity of the device, with performance comparable to a conventional stand‐alone nanopore setup. However, the microfluidic–nanopore setup is superior in terms of ease of use. To showcase the utility of the device, single molecule detection of a DNA polymerase chain reaction product, after magnetic bead DNA separation, is accomplished on‐chip.

中文翻译：

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具有多层流体和片上生物测定/纯化室的固态硬微流体-纳米孔生物传感器

固态纳米孔是一种用于核酸和蛋白质表征的新兴生物传感器。为了在临床环境中使用，固态纳米孔传感需要样品制备和纯化、流体处理、加热元件、电噪声绝缘体和电读出检测器，所有这些都阻碍了其转化为护理点诊断设备。描述了一种基于微流体的独立纳米孔装置，它将生物测定反应/纯化室与固态纳米孔传感器相结合。该微流体装置由耐高温/耐溶剂的 Zeonex 塑料组成，通过微加工和热粘合形成，可同时使用热调节器和磁控制器。通过微流体通道和腔室的流体控制通过流体端口选择阀进行控制，并允许多达八种不同的解决方案。电噪声测量和 DNA 易位实验证明了该装置的完整性，其性能可与传统的独立纳米孔装置相媲美。然而，微流控-纳米孔设置在易用性方面更为优越。为了展示该设备的实用性，在磁珠 DNA 分离后，在芯片上完成了 DNA 聚合酶链式反应产物的单分子检测。