Soils contaminated by toxic metallic elements from agricultural activities raise grave concern about their potential risk to human health through direct intake, bioaccumulation through the food chain, and their impacts on ecological systems. We have measured here the lipid and protein oxidation status and used metabolomic methodologies to identify and characterize the changes caused by metal pollution exposure in the digestive glands and gills of Procambarus clarkii, the red swamp crayfish. Specimens captured at two sites with intensive agriculture practices using diverse types of agrochemicals, located in the borders of Doñana Natural Park, were compared to ones caught in the core of the Park, a proven non-polluted place. As a highly metabolically active organ, the digestive gland accumulated more metallic elements than the gills and was consequently more affected at the metabolic level. Results also indicate that chronic pollution exposure generates oxidative stress and mitochondrial dysfunction that imposes a metabolic shift to enhanced aerobic glycolysis and lipid metabolism alteration. The integration of metabolomics with previous proteomic data gives a comprehensive vision of the metabolic disorders caused by chronic metal exposure to P. clarkii and identifies potential biomarkers useful for routine risk assessment of the aquatic ecosystems health.

农业活动中被有毒金属元素污染的土壤引起了人们的严重关切，这些土壤通过直接摄入对人体健康的潜在威胁，通过食物链的生物蓄积及其对生态系统的影响。我们在这里测量了脂质和蛋白质的氧化状态，并使用了代谢组学方法来鉴定和表征金属污染在克氏原螯虾的消化腺和g中引起的变化。，红色沼泽小龙虾。将位于Doñana自然公园边界的两个采用多种农用化学品进行集约化农业实践的地点捕获的标本与在公园核心地带捕获的标本进行了比较，该地区已被证明是无污染的。作为高代谢活性器官，消化腺比than积累更多的金属元素，因此在代谢水平上受到的影响更大。结果还表明，长期污染暴露会产生氧化应激和线粒体功能障碍，从而迫使新陈代谢转变为需氧糖酵解和脂质代谢改变。代谢组学与以前的蛋白质组学数据的整合提供了由慢性金属暴露于克拉克疟原虫引起的代谢紊乱的全面视野 并确定有助于对水生生态系统健康进行例行风险评估的潜在生物标志物。