Peanut suffer from a number of fungal and bacterial pathogens, while plant endophytes were considered excellent candidates as biocontrol agents. In this study, the peanut endophytic bacterium LDO2 was evaluated for the potential of peanut pathogens inhibition and growth-promotion, and the genetic mechanisms were explored by genome mining. Strain LDO2 significantly inhibited the growth of peanut pathogenic fungi and pathogenic bacteria, and specifically, it showed pronounced inhibition on mycelia growth of Aspergillus flavus mycelia and caused mycelial deformity. Gene clusters responsible for antifungal metabolites (fengycin, surfactin, bacilysin) and antibacterial metabolites (butirosin, bacillaene, difficidin, macrolactin, surfactin, bacilysin) were identified. Strain LDO2 also exhibited several growth-promoting related features including phosphate solubilization, siderophore production and growth promotion of peanut root. Genes associated with plant growth promotion were also identified and analyzed, as well as genes related to secreted proteins. These findings suggested that this peanut endophyte could be a potential biocontrol agent in peanut production and a source of antimicrobial compounds for further exploitation.

花生患有多种真菌和细菌病原体，而植物内生菌则被认为是生物防治剂的极佳候选者。在这项研究中，评估了花生内生细菌LDO2抑制花生病原菌和促进其生长的潜力，并通过基因组挖掘探索了其遗传机制。LDO2菌株显着抑制花生致病真菌和致病菌的生长，特别是它对黄曲霉菌丝体的生长具有明显的抑制作用。菌丝体引起菌丝畸形。确定了负责抗真菌代谢产物（丰霉素，表面活性素，杆菌肽溶酶）和抗菌代谢产物（丁螺旋素，杆菌素，艰难梭菌素，大内泌素，表面活性素，杆菌肽溶酶）的基因簇。LDO2菌株还表现出几种促进生长的相关特征，包括磷酸盐增溶，铁载体生成和花生根的生长促进。还鉴定并分析了与植物生长促进相关的基因，以及与分泌蛋白有关的基因。这些发现表明，该花生内生菌可能是花生生产中潜在的生物防治剂，并且是进一步开发抗菌化合物的来源。