Harmful Algae ( IF 5.5 ) Pub Date : 2018-10-06 , DOI: 10.1016/j.hal.2018.06.004 Nancy I. Lewis , Stephen S. Bates , Michael A. Quilliam
The commercial demand for domoic acid (DA), the phycotoxin responsible for Amnesic Shellfish Poisoning, is currently met by extraction from a diminishing supply of stockpiled contaminated mussels (Mytilus edulis). As this supply becomes scarce, a more reliable source is needed. Purification of the toxin from an algal source would be easier and more economical than from shellfish tissue if algal growth and yield of toxin were maximized. This project was initiated to determine if DA could be produced using large-scale semi-continuous algal cultures, which should reduce labour and shorten the time required for biomass production. Pseudo-nitzschia multiseries was grown in 300-L fibreglass photobioreactors called a Brite-Box™. The effect of temperature and nutrient depletion on the yield of DA by P. multiseries was examined. A decline in maximum cell number without a substantial increase in cellular DA was associated with increased temperature. Maximum total cellular DA (8.8 pg cell−1) was achieved at 20 °C. Semi-continuous culture of P. multiseries is accompanied by increasing amounts of DA lost to the medium. The process was deemed to be feasible for growing P. multiseries but methods to recover this extracellular DA are necessary for this process to be economical.
中文翻译:
从多假拟南芥的大规模培养生产二氢氰酸的可行性研究
目前,通过减少储存的受污染贻贝(Mytilus edulis)的供应量,可以满足对引起遗忘性贝类中毒的藻毒素藻酸(DA)的商业需求。由于这种供应变得稀缺,因此需要更可靠的来源。如果使藻类的生长和毒素的产量最大化,则从藻类来源纯化毒素比从贝类组织中纯化更为容易和经济。启动该项目的目的是确定DA是否可以使用大规模的半连续藻类培养产生,这将减少劳动力并缩短生物质生产所需的时间。拟南芥多系列在称为Brite-Box™的300升玻璃纤维光生物反应器中生长。研究了温度和养分耗竭对多系拟青霉DA产量的影响。最大细胞数的减少没有细胞DA的显着增加与温度升高有关。在20℃下获得最大的总细胞DA(8.8pg细胞-1)。多系列疟原虫的半连续培养伴随着丢失到培养基中的DA量的增加。该方法被认为对于生长多系列疟原虫是可行的,但是回收该细胞外DA的方法对于该方法是经济的是必要的。