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Modulation of hepatic lipidome by rhodioloside in high-fat diet fed apolipoprotein E knockout mice.
Phytomedicine ( IF 6.7 ) Pub Date : 2018-09-27 , DOI: 10.1016/j.phymed.2018.09.225 Shi-Yuan Wen 1 , Yan-Yan Chen 1 , Jia-Xi Lu 2 , Qian-Qian Liang 2 , Hong Shi 2 , Qian Wu 3 , Zhi-Hong Yao 4 , Yan Zhu 2 , Miao-Miao Jiang 2
Phytomedicine ( IF 6.7 ) Pub Date : 2018-09-27 , DOI: 10.1016/j.phymed.2018.09.225 Shi-Yuan Wen 1 , Yan-Yan Chen 1 , Jia-Xi Lu 2 , Qian-Qian Liang 2 , Hong Shi 2 , Qian Wu 3 , Zhi-Hong Yao 4 , Yan Zhu 2 , Miao-Miao Jiang 2
Affiliation
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BACKGROUND
Rhodioloside is a glucoside of tyrosol isolated from Rhodiola rosea. However, its regulating effect on hepatic dyslipidemia of atherogenic mice has rarely been studied.
PURPOSE
The specific aims of current study included to clarify lipidomic perturbation in liver tissues of apolipoprotein E deficient (apoE-/-) mice fed with high-fat diet, and to examine the effects of rhodioloside against atherosclerosis and dyslipidemia.
STUDY DESIGN
The comparisons of hepatic lipidome were executed between wide type (WT) mice fed with normal diet (NDC) and apoE-/- mice fed with high-fat diet (Model), WT mice fed with high-fat diet (HFDC) versus the model mice, as well as the model mice versus rhodioloside-treated atherosclerotic mice.
METHODS
Ultra high performance liquid chromatography coupled with a Q exactive hybrid quadrupole-orbitrap mass spectrometry (UPLC-MS/MS) was employed to provide an unbiased and simultaneous measurement of individual lipid species in liver tissues.
RESULTS
Multivariate statistical analysis derived from LC-MS spectra revealed that high-fat diet and apoE deficiency caused a series of disturbances on glyerolipid metabolism, glycerophospholipid metabolism and sphingolipid metabolism. Rhodioloside administration showed atheroprotective effects on the apoE-/- mice with regulating the levels of 1 phosphatidylcholine, 2 phosphatidylserines, 5 alkyldiacylglycerols and 3 alkenyldiacylglycerols back to normal. In particular, PC (4:0/15:0) was positively associated with high-density lipoprotein cholesterol in blood, both of which could be ameliorated by rhodioloside.
CONCLUSION
Our results identified the abnormal hepatic lipids in atherosclerosis progression that could efficiently improved by rhodioloside. These lipids contributed to biological understanding of atherogenic dyslipidemia in liver and could also served as sensitive indicators for drug target screening.
中文翻译：
红景天苷对高脂饮食载脂蛋白E基因敲除小鼠肝脂质组的调节。
背景技术红景天苷是从玫瑰红景天中分离出的酪醇的葡糖苷。然而，很少研究其对动脉粥样硬化小鼠肝血脂异常的调节作用。目的当前研究的具体目标包括阐明高脂饮食喂养的载脂蛋白E缺乏症（apoE-/-）小鼠肝脏组织中的脂质组微扰，并检查杜鹃花苷对动脉粥样硬化和血脂异常的影响。研究设计在喂食普通饮食（NDC）的宽型（WT）小鼠和喂高脂饮食（模型）的apoE-/-小鼠，喂高脂饮食（HFDC）的WT小鼠之间进行肝脂质组的比较与模型小鼠相比，以及模型小鼠与经红景天苷治疗的动脉粥样硬化小鼠之间的比较。方法采用超高效液相色谱与Q精确混合四极杆-轨道质谱（UPLC-MS / MS）结合使用，可无偏且同时测量肝脏组织中的单个脂质种类。结果LC-MS光谱的多变量统计分析表明，高脂饮食和apoE缺乏会引起一系列的脂质代谢，甘油磷脂代谢和鞘脂代谢紊乱。红景天苷的给药对apoE-/-小鼠具有抗动脉粥样硬化的作用，可调节1磷脂酰胆碱，2磷脂酰丝氨酸，5烷基二酰基甘油和3烯基二酰基甘油的水平。特别是PC（4：0/15：0）与血液中的高密度脂蛋白胆固醇呈正相关，红景天苷可以改善这两个方面。结论我们的研究结果确定了可以通过红景天苷有效改善动脉粥样硬化进展中异常的肝脂质。这些脂质有助于对肝中动脉粥样硬化血脂异常的生物学理解，也可以用作药物靶标筛选的敏感指标。
更新日期：2018-09-27
中文翻译：
红景天苷对高脂饮食载脂蛋白E基因敲除小鼠肝脂质组的调节。
背景技术红景天苷是从玫瑰红景天中分离出的酪醇的葡糖苷。然而，很少研究其对动脉粥样硬化小鼠肝血脂异常的调节作用。目的当前研究的具体目标包括阐明高脂饮食喂养的载脂蛋白E缺乏症（apoE-/-）小鼠肝脏组织中的脂质组微扰，并检查杜鹃花苷对动脉粥样硬化和血脂异常的影响。研究设计在喂食普通饮食（NDC）的宽型（WT）小鼠和喂高脂饮食（模型）的apoE-/-小鼠，喂高脂饮食（HFDC）的WT小鼠之间进行肝脂质组的比较与模型小鼠相比，以及模型小鼠与经红景天苷治疗的动脉粥样硬化小鼠之间的比较。方法采用超高效液相色谱与Q精确混合四极杆-轨道质谱（UPLC-MS / MS）结合使用，可无偏且同时测量肝脏组织中的单个脂质种类。结果LC-MS光谱的多变量统计分析表明，高脂饮食和apoE缺乏会引起一系列的脂质代谢，甘油磷脂代谢和鞘脂代谢紊乱。红景天苷的给药对apoE-/-小鼠具有抗动脉粥样硬化的作用，可调节1磷脂酰胆碱，2磷脂酰丝氨酸，5烷基二酰基甘油和3烯基二酰基甘油的水平。特别是PC（4：0/15：0）与血液中的高密度脂蛋白胆固醇呈正相关，红景天苷可以改善这两个方面。结论我们的研究结果确定了可以通过红景天苷有效改善动脉粥样硬化进展中异常的肝脂质。这些脂质有助于对肝中动脉粥样硬化血脂异常的生物学理解，也可以用作药物靶标筛选的敏感指标。
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