Four hundred and fifty bacteria were evaluated for antagonistic activity against bacterial soft rot of potato caused by Pectobacterium carotovorum sp strain II16. A strain Ar10 exhibiting potent antagonist activity has been identified as Bacillus amyloliquefaciens on the basis of biochemical and molecular characterization. Cell free supernatant showed a broad spectrum of antibacterial activity against human and phytopathogenic bacteria in the range of 10-60 AU/mL. Incubation of P. carotovorum cells with increasing concentrations of the antibacterial compound showed a killing rate of 94.8 and 96% at MIC and 2xMIC respectively. In addition, the antibacterial agent did not exert haemolytic activity at the active concentration and has been preliminary characterized by TLC and GC–MS as a glycolipid compound.

Treatment of potato tubers with strain Ar10 for 72 h significantly reduced the severity of disease symptoms (100 and 85.05% reduction of necrosis deep / area and weight loss respectively). The same levels in disease symptoms severity was also recorded following treatment of potato tubers with cell free supernatant for 1 h. Data suggest that protection against potato soft rot disease may be related to glycolipid production by strain Ar10. The present study affords new alternatives for anti-Pectobacterium carotovorum bioactive compounds against the soft rot disease of potato.

评价了450个细菌对由胡萝卜软腐菌属菌株II16引起的马铃薯的细菌软腐的拮抗活性。基于生化和分子表征，已将表现出有效拮抗剂活性的菌株Ar10鉴定为解淀粉芽孢杆菌。无细胞上清液在10-60 AU / mL的范围内显示出对人类和植物致病菌的广泛抗菌活性。胡萝卜假单胞菌的孵化抗菌化合物浓度增加的细胞在MIC和2xMIC处的杀灭率分别为94.8和96％。此外，该抗菌剂在有效浓度下不发挥溶血活性，并已通过TLC和GC-MS初步鉴定为糖脂化合物。

用Ar10菌株处理马铃薯块茎72小时可显着降低疾病症状的严重程度（坏死深度/面积和体重减轻分别减少100和85.05％）。用无细胞上清液处理马铃薯块茎1小时后，也记录了相同水平的疾病症状严重程度。数据表明，针对马铃薯软腐病的保护作用可能与Ar10菌株产生糖脂有关。本研究为抗马铃薯软腐病菌生物活性化合物提供了针对马铃薯软腐病的新替代品。