Entomopathogenic fungi (EPF) can be established as endophytes in the host plants to offer a long-term preventive measure for pests and diseases. This practice serves as a better alternative to the common practice of periodic direct application of EPF on plants or the target pests as a short-term defense strategy against pests and diseases. These fungal endophytes, aside from their role in pests and diseases prevention, also act as plant growth promoters. Several fungal endophytes have been associated with improvement in plant height, dry and wet weight and other growth parameters. However, many limiting factors have been identified as mitigating the successful colonization of the host plants by EPF. The inoculation methods used have been identified as one, but sadly, this has received little or less attention.

Some previous studies carried out comparison between various artificial inoculation methods; foliar application, seedling dipping, soil drenching, seed inoculation, direct injection and others. In separate studies, some authors had suggested different application methods that are best suitable for certain fungal entomopathogens. For instance, leaf inoculation with conidial suspensions was suggested to be the best inoculation method for Beauveria bassiana in sorghum, stem injection was suggested as the most suitable for coffee, while, root dipping method proved the most successful for B. bassiana colonization of tomato plants for the management of Helicoverpa armigera.

Here, we discussed entomopathogenic fungal endophytes as bio-control agents, plant growth promoters and highlighted the effect of various artificial inoculation methods on their endophytic colonization of the host plants.

可以将病原性真菌（EPF）建立为宿主植物中的内生菌，从而为病虫害提供长期的预防措施。这种做法可以更好地替代将EPF定期直接应用于植物或目标害虫的常规做法，将其作为针对病虫害的短期防御策略。这些真菌内生菌除了在病虫害预防中的作用外，还可以作为植物的生长促进剂。几种真菌内生菌与植物高度，干重和湿重以及其他生长参数的改善有关。然而，许多限制因素已被确定为减轻了EPF对宿主植物的成功定植。所使用的接种方法已被确定为一种，但可悲的是，这种方法很少受到关注。

先前的一些研究对各种人工接种方法进行了比较。叶面施用，幼苗浸入，土壤浸湿，种子接种，直接注射等。在单独的研究中，一些作者提出了最适合某些真菌昆虫病原体的不同施用方法。例如，建议用分生孢子悬浮液接种叶是高粱白僵菌的最佳接种方法，建议茎注射是最适合于咖啡的，而根浸法被证明最能成功地用于球茎白僵菌在番茄植物上的定殖。用于棉铃虫的管理。

在这里，我们讨论了致病性真菌内生菌作为生物防治剂，植物生长促进剂，并强调了各种人工接种方法对其宿主植物内生菌落的影响。